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验证数据展示

  • WB analysis using 30845-1-AP

    WB analysis using 30845-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30845-1-AP (FAM136A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 30845-1-AP

    IF Staining of U2OS using 30845-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FAM136A antibody (30845-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 30845-1-AP

    IHC staining of human ovary cancer using 30845-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30845-1-AP (FAM136A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAM136A Polyclonal antibody

FAM136A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30845-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Protein FAM136A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU2OS cells, A549 cells, HeLa cells, Jurkat cells, MCF-7 cells, mouse testis tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

30845-1-AP targets FAM136A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 FAM136A fusion protein Ag34058 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 136, member A
别名 Protein FAM136A
观测分子量15 kDa
GenBank蛋白编号BC014975
基因名称 FAM136A
Gene ID (NCBI) 84908
RRIDAB_3086420
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96C01
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM136A is an evolutively conserved nuclear gene encoding a mitochondrial protein whose specific function is unknown, but which is commonly found in neurosensory epithelial cells, where it plays a role in the electron transport chain of respiration (PMID: 37461313). FAM136A, with a molecular mass of 16-kDa, is normally expressed in the cytoplasm and has been linked to familial Meniere's disease.

实验方案

Product Specific Protocols
WB protocol for FAM136A antibody 30845-1-APDownload protocol
IHC protocol for FAM136A antibody 30845-1-APDownload protocol
IF protocol for FAM136A antibody 30845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

FEBS Open Bio

FAM136A depletion induces mitochondrial stress and reduces mitochondrial membrane potential and ATP production

Authors - Yushi Otsuka
View Article