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验证数据展示

  • WB analysis using 29917-1-AP

    WB analysis using 29917-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29917-1-AP (FAM114A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 29917-1-AP

    IF Staining of HeLa using 29917-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FAM114A2 antibody (29917-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FAM114A2 Polyclonal antibody

FAM114A2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29917-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IF/ICC, ELISA

别名

133K02, C5orf3, FAM114A2, Protein FAM114A2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, MOLT-4 cells, U-251 cells, mouse testis tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29917-1-AP targets FAM114A2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原

CatNo: Ag31963

Product name: Recombinant human FAM114A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 255-505 aa of BC011414

Sequence: MLSQESEIKVKSILNSLSGEELETLKVELEQLKETFSLAEFCEEEEEEKKGDEDFTKDITELFSQLHVSSKPEKLARARNTAHEWIRKSLTKPLAENEEGEKQSEAENTEQVNKNSIEDIHAFAIRSLAELTACSIELFHKTAALVLHGRKQEVTAIERSQTLSQMTIVLCKELSSLSKEFTTCLTTAGVKEMADVLNPLITAVFLEASNSASYIQDAFQLLLPVLEISLIENKIESHRHELQGQKPLLEH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 114, member A2
别名 133K02, C5orf3, FAM114A2, Protein FAM114A2
观测分子量55 kDa
GenBank蛋白编号BC011414
基因名称 FAM114A2
Gene ID (NCBI) 10827
RRIDAB_3086188
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRY5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FAM114A2, also known as C5orf3, belongs to the nervous overexpress protein family and has been implicated in neuronal cell development. FAM114A2 a human testis enrich gene and testis sperm-binding protein, is highly conserved in mammals. The observed band size is around 55 kDa.

实验方案

Product Specific Protocols
IF protocol for FAM114A2 antibody 29917-1-APDownload protocol
WB protocol for FAM114A2 antibody 29917-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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