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EXOSC6 Polyclonal antibody

EXOSC6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 30685-1-AP

Print datasheet

Synonyms

EAP4, EXOSC6, exosome component 6, hMtr3, hMtr3p, MTR3, Mtr3p, p11

验证数据展示

  • WB analysis using 30685-1-AP

    WB analysis using 30685-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30685-1-AP (EXOSC6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHaCaT cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30685-1-AP targets EXOSC6 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen EXOSC6 fusion protein Ag33513 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name exosome component 6
Synonyms EAP4, EXOSC6, exosome component 6, hMtr3, hMtr3p, MTR3, Mtr3p, p11
Calculated Molecular Weight28kd
Observed Molecular Weight28-32 kDa
GenBank Accession NumberNM_058219
Gene Symbol EXOSC6
Gene ID (NCBI) 118460
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5RKV6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EXOSC6 (exosome component 6), also known as p11 or MTR3. The calculated molecular weight of EXOSC6 is 28 kDa. And it has low tissue specificity. The gene product constitutes one of the subunits of the multisubunit particle called exosome, which mediates mRNA degradation. It is also a component of the RNA exosome complex (PMID: 29906447). The composition of human exosome is similar to its yeast counterpart. EXOSC6 is homologous to the yeast Mtr3 protein. Its exact function is not known, however, it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs), but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own, but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs, thereby promoting their rapid degradation.

实验方案

Product Specific Protocols
WB protocol for EXOSC6 antibody 30685-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols