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验证数据展示

  • WB analysis using 68374-1-Ig (same clone as 68374-1-PBS)

    WB analysis using 68374-1-Ig (same clone as 68374-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68374-1-Ig (ESYT2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68374-1-Ig (same clone as 68374-1-PBS)

    WB analysis of HeLa using 68374-1-Ig (same clone as 68374-1-PBS)

    WB result of FAM62B antibody (68374-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM62B transfected HeLa cells. This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 68374-1-Ig (same clone as 68374-1-PBS)

    IF Staining of MCF-7 using 68374-1-Ig (same clone as 68374-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ESYT2 antibody (68374-1-Ig, Clone: 1B12G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68374-1-PBS in a different storage buffer formulation.

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ESYT2 Monoclonal antibody, PBS Only

ESYT2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68374-1-PBS

产品说明书

CloneNo. 1B12G2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

FAM62B, Chr2Syt, E Syt2, E-Syt2, Extended synaptotagmin 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FAM62B Ready-To-Use IHC Kit. FAM62B primary antibody included.

产品信息

68374-1-PBS targets ESYT2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20440

Product name: Recombinant human FAM62B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 559-773 aa of BC013957

Sequence: NPVVQMSVGHKAQESKIRYKTNEPVWEENFTFFIHNPKRQDLEVEVRDEQHQCSLGNLKVPLSQLLTSEDMTVSQRFQLSNSGPNSTIKMKIALRVLHLEKRERPPDHQHSAQVKRPSVSKEGRKTSIKSHMSGSPGPGGSNTAPSTPVIGGSDKPGMEEKAQPPEAGPQGLHDLGRSSSSLLASPGHISVKEPTPSIASDISLPIATQELRQRL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 family with sequence similarity 62 (C2 domain containing) member B
别名 FAM62B, Chr2Syt, E Syt2, E-Syt2, Extended synaptotagmin 2
计算分子量 921 aa, 102 kDa
观测分子量90-100 kDa
GenBank蛋白编号BC013957
基因名称 ESYT2
Gene ID (NCBI) 57488
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDA0FGR8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ESYT2, also named FAM62B, is one of the three-member family of Extended Synaptotagmins that were recently shown to be implicated in the formation of endoplasmic reticulum (ER)-plasma membrane (PM) junctions and in the Ca(2+) dependent regulation of these junctions.

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