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验证数据展示

  • WB analysis using 31432-1-AP

    WB analysis using 31432-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31432-1-AP (ERMN antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 31432-1-AP

    IF Staining of mouse brain using 31432-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ERMN antibody (31432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 31432-1-AP

    IF Staining of rat brain using 31432-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ERMN antibody (31432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ERMN Polyclonal antibody

ERMN Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 31432-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

KIAA1189, Juxtanodin, JN, Ermin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat brain tissue, rat cerebellum tissue
Positive IF-P detected inrat brain tissue, mouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31432-1-AP targets ERMN in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35648

Product name: Recombinant human ERMN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-257 aa of BC026345

Sequence: MTDVPATFTQAECNGDKPPENGQQTITKISEELTDVDSPLPHYRVEPSLEGALTKGSQEERRKLQGNMLLNSSMEDKMLKENPEEKLFIVHKAITDLSLQETSADEMTFREGHQWEKIPLSGSNQEIRRQKERITEQPLKEEEDEDRKNKGHQAAEIEWLGFRKPSQADMLHSKHDEEQKVWDEEIDDDDDDNCNNDEDEVRVIEFKKKHEEVSQFKEEGDASEDSPLSSASSQAVTPDEQPTLGKKSDISRNAYSR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ermin, ERM-like protein
别名 KIAA1189, Juxtanodin, JN, Ermin
观测分子量 45 kDa
GenBank蛋白编号BC026345
基因名称 ERMN
Gene ID (NCBI) 57471
RRIDAB_3669981
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8TAM6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERMN is associated with cytoskeletal rearrangements and the stability of myelin sheath. A study has shown that ERMN is expressed specifically in oligodendrocytes in the central nervous system (CNS), and is involved in the formation of myelin (PMID: 20934411). The predicted MW of ERMN is 33 KDa, while western blot analyses detected a 45-50 kDa single band protein as a result of phosphorylation modification.

实验方案

Product Specific Protocols
IF protocol for ERMN antibody 31432-1-APDownload protocol
WB protocol for ERMN antibody 31432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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