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验证数据展示

  • WB analysis of HEK-293 using 16639-1-AP (same clone as 16639-1-PBS)

    WB analysis of HEK-293 using 16639-1-AP (same clone as 16639-1-PBS)

    WB result of EPLIN antibody (16639-1-AP, 1:1000) with si-Control and si-EPLIN transfected HEK293 cells. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 16639-1-AP (same clone as 16639-1-PBS)

    IHC staining of human prostate cancer using 16639-1-AP (same clone as 16639-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16639-1-AP (EPLIN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 16639-1-AP (same clone as 16639-1-PBS)

    IP experiment of mouse liver using 16639-1-AP (same clone as 16639-1-PBS)

    IP result of anti-EPLIN (IP:16639-1-AP, 4ug; Detection:16639-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 16639-1-AP (same clone as 16639-1-PBS)

    IF Staining of PC-3 using 16639-1-AP (same clone as 16639-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using EPLIN antibody (16639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • WB analysis of HUVEC using 16639-1-AP (same clone as 16639-1-PBS)

    WB analysis of HUVEC using 16639-1-AP (same clone as 16639-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 16639-1-AP (EPLIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 16639-1-AP (same clone as 16639-1-PBS)

    IF Staining of HepG2 using 16639-1-AP (same clone as 16639-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPLIN antibody (16639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16639-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

EPLIN Polyclonal antibody, PBS Only

EPLIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 16639-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

LIMA1, SREBP3, PP624, LIM domain and actin-binding protein 1, LIM domain and actin binding 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

16639-1-PBS targets EPLIN in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EPLIN fusion protein Ag9994 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LIM domain and actin binding 1
别名 LIMA1, SREBP3, PP624, LIM domain and actin-binding protein 1, LIM domain and actin binding 1
计算分子量 85 kDa
观测分子量 90 kDa, 110 kDa
GenBank蛋白编号BC001247
基因名称 EPLIN
Gene ID (NCBI) 51474
RRIDAB_2136657
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHB6
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. The 110 kDa EPLIN-β isoform represents the full-length protein and the 90 kDa EPLIN-α isoform lacks the amino-terminal 160 amino acids. EPLIN-a expression is often down-regulated in cancerous cells and tissues. (PMID: 10806352, 31644899)