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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of mouse testis using 13946-1-AP (same clone as 13946-1-PBS)

    IHC staining of mouse testis using 13946-1-AP (same clone as 13946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13946-1-AP (EMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13946-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 13946-1-AP (same clone as 13946-1-PBS)

    WB analysis of HeLa using 13946-1-AP (same clone as 13946-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13946-1-PBS in a different storage buffer formulation.

EMB Polyclonal antibody, PBS Only

EMB Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13946-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

13946-1-PBS targets EMB in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5031

Product name: Recombinant human EMB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-253 aa of BC059398

Sequence: MRALPGLLEARARTPRLLLLQCLLAAARPSSADGSAPDSPFTSPPLREEIMANNFSLESHNISLTEHSSMPVEKNITLERPSNVNLTCQFTTSGDLNAVNVTWKKDGEQLENNYLVSATGSTLYTQYRFTIINSKQMGSYSCFFREEKEQRGTFNFKVPELHGKNKPLISYVGDSTVLTCKCQNCFPLNWTWYSSNGSVKVPVGVQMNKYVINGTYANETKLKITQLLEEDGESYWCRALFQLGESEEHIELV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 embigin homolog (mouse)
别名
计算分子量 37 kDa
观测分子量 70 kDa
GenBank蛋白编号BC059398
基因名称 EMB
Gene ID (NCBI) 133418
RRIDAB_2877993
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PCB8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EMB, also named as Embigin, is a 327 amino acid protein, which contains two Ig-like V-type (immunoglobulin-like) domains. EMB as a membrane protein may unction as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues. EMB is a transmembrane glycoprotein with a protein component of 34 kDa, but western blot analysis of embigin in mouse soleus muscles detects a glycolyated full-length protein with an molecular mass of 70 kDa.(PMID: 19164284)