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验证数据展示

  • WB analysis using 26557-1-AP

    WB analysis using 26557-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26557-1-AP (ECT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 26557-1-AP

    WB analysis using 26557-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26557-1-AP (ECT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 26557-1-AP

    IP experiment of K-562 using 26557-1-AP

    IP result of anti-ECT2 (IP:26557-1-AP, 4ug; Detection:26557-1-AP 1:6000) with K-562 cells lysate 2400 ug.

  • FC experiment of A549 using 26557-1-AP

    FC experiment of A549 using 26557-1-AP

    1x10^6 A549 cells were intracellularly stained with 0.25 ug ECT2 Polyclonal antibody (26557-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of A549 using 26557-1-AP

    IF Staining of A549 using 26557-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ECT2 antibody (26557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IF Staining of NIH/3T3 using 26557-1-AP

    IF Staining of NIH/3T3 using 26557-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ECT2 antibody (26557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ECT2 Polyclonal antibody

ECT2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 26557-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), IP, ELISA

别名

Protein ECT2, Epithelial cell-transforming sequence 2 oncogene

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293T cells, K-562 cells
Positive IP detected inK-562 cells
Positive IF/ICC detected inA549 cells, NIH/3T3 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26557-1-AP targets ECT2 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human
免疫原 ECT2 fusion protein Ag24836 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epithelial cell transforming sequence 2 oncogene
别名 Protein ECT2, Epithelial cell-transforming sequence 2 oncogene
观测分子量 100 kDa
GenBank蛋白编号BC112086
基因名称 ECT2
Gene ID (NCBI) 1894
RRIDAB_3669547
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H8V3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Epithelial cell transforming sequence 2 (Ect2) is a guanine nucleotide exchange factor (GEF) for the Rho family GTPases, Rac1, RhoA and Cdc42. Ect2 catalyzes the exchange of GDP for GTP, thereby activating the Rho GTPases toward their downstream effectors  (PMID: 35069543). The cellular localization of Ect2 is cell cycle dependent. Ect2 is localized predominantly in the nucleus during interphase due to two functional NLS sequences found within its central hinge region.1 At the onset of mitosis, Ect2 is dispersed throughout the cytoplasm where it associates with the mitotic spindle at metaphase, the cleavage furrow during telophase, and the mid-body during cytokinesis (PMID: 35069543). Besides normal cellular activity, ECT2 also participates in malignant transformation, tumor initiation, and metastasis (PMID: 36572949).

实验方案

Product Specific Protocols
WB protocol for ECT2 antibody 26557-1-APDownload protocol
IF protocol for ECT2 antibody 26557-1-APDownload protocol
IP protocol for ECT2 antibody 26557-1-APDownload protocol
FC protocol for ECT2 antibody 26557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols