验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| ChIP | See 2 publications below |
产品信息
13425-1-AP targets E2F8 in WB, IP, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, goat |
| 免疫原 |
CatNo: Ag4216 Product name: Recombinant human E2F8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 78-508 aa of BC028244 Sequence: MAQLAAICKMQLEEQSSESRQKVKVQLARSGPCKPVAPLDPPVNAEMELTAPSLIQPLGMVPLIPSPLSSAVPLILPQAPSGPSYAIYLQPTQAHQSVTPPQGLSPTVCTTHSSKATGSKDSTDATTEKAANDTSKASASTRPGSLLPAPERQGAKSRTREPAGERGSKRASMLEDSGSKKKFKEDLKGLENVSATLFPSGYLIPLTQCSSLGAESILSGKENSSALSPNHRIYSSPIAGVIPVTSSELTAVNFPSFHVTPLKLMVSPTSVAAVPVGNSPALASSHPVPIQNPSSAIVNFTLQHLGLISPNVQLSASPGSGIVPVSPRIESVNVAPENAGTQQGRATNYDSPVPGQSQPNGQSVAVTGAQQPVPVTPKGSQLVAESFFRTPGGPTKPTSSSCMDFEGANKTSLGTLFVPQRKLEVSTEDVH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | E2F transcription factor 8 |
| 别名 | E2F 8, E2F transcription factor 8, E2F-8, Transcription factor E2F8 |
| 计算分子量 | 867 aa, 94 kDa |
| 观测分子量 | 105 kDa |
| GenBank蛋白编号 | BC028244 |
| 基因名称 | E2F8 |
| Gene ID (NCBI) | 79733 |
| RRID | AB_2097273 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A0AVK6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
E2F8 is one E2F transcription factor that is essential for orchestrating expression of genes required for cell cycle progression, proliferation, apoptosis and differentiation. E2F8 shows a high degree of resemblance to E2F7 and shares the unique structure of E2F7 by having two distinct domains exhibiting a high degree of similarity to the DNA-binding domain of the E2F familyTogether with E2F7, they possess two DNA-binding domains that are predicted to interact with each other. E2F8 binds consensus E2F sites in a DP-independent manner and represses transcription of E2F-regulated promoters. Ectopic expression of E2F8 inhibits cellular proliferation. The calcualted molecular weight of E2F8 is 94 kDa, but modified E2F8 is about 105 kDa. (PMID: 15897886)
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for E2F8 antibody 13425-1-AP | Download protocol |
| WB protocol for E2F8 antibody 13425-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Discov Molecular mechanism underlying miR-204-5p regulation of adipose-derived stem cells differentiation into cells from three germ layers | ||
Adv Sci (Weinh) Genetic Aberrations and Interaction of NEK2 and TP53 Accelerate Aggressiveness of Multiple Myeloma | ||
Int J Toxicol Transcription Factor E2F8 Promotes Cisplatin Resistance in Hepatocellular Carcinoma by Regulating DNA Damage via NUSAP1 | ||
Cell Death Discov Carmofur prevents cell cycle progression by reducing E2F8 transcription in temozolomide-resistant glioblastoma cells |
