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验证数据展示

  • WB analysis using 29814-1-AP (same clone as 29814-1-PBS)

    WB analysis using 29814-1-AP (same clone as 29814-1-PBS)

    Human placenta tissue were subjected to SDS PAGE followed by western blot with 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29814-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 29814-1-AP (same clone as 29814-1-PBS)

    IHC staining of mouse kidney using 29814-1-AP (same clone as 29814-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29814-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cytochrome P450 antibody included
  • IHC staining of rat kidney using 29814-1-AP (same clone as 29814-1-PBS)

    IHC staining of rat kidney using 29814-1-AP (same clone as 29814-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29814-1-AP (Cytochrome P450 Reductase antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29814-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cytochrome P450 antibody included
  • WB analysis of HepG2 using 29814-1-AP (same clone as 29814-1-PBS)

    WB analysis of HepG2 using 29814-1-AP (same clone as 29814-1-PBS)

    WB result of Cytochrome P450 Reductase antibody (29814-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cytochrome P450 Reductase transfected HepG2 cells. This data was developed using the same antibody clone with 29814-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Cytochrome P450 Reductase Polyclonal antibody, PBS Only

Cytochrome P450 Reductase Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29814-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

POR, P450R, CYPOR, CPR

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® Cytochrome P450 Ready-To-Use IHC Kit. Cytochrome P450 primary antibody included.

产品信息

29814-1-PBS targets Cytochrome P450 Reductase in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag30899

Product name: Recombinant human POR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 43-216 aa of NM_000941

Sequence: LFRKKKEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 P450 (cytochrome) oxidoreductase
别名 POR, P450R, CYPOR, CPR
计算分子量 77 kDa
观测分子量77 kDa
GenBank蛋白编号NM_000941
基因名称 Cytochrome P450 Reductase
Gene ID (NCBI) 5447
RRIDAB_3086171
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16435
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytochrome P450 Reductase (CYPOR) consists of the flavin mononucleotide (FMN) domain and the flavin adenine dinucleotide (FAD) domain, which bind the corresponding cofactors and transfer electrons from NADPH via FAD and FMN (PMID: 33498551). CYPOR is an essential component of the microsomal P450 mixed-function oxidase system and is required for electron transfer from NADPH to cytochromes P450 in microsomes. The calculated molecular weight of CYPOR is 77 kDa.

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