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Cxcl15 Polyclonal antibody

Cxcl15 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, IP, ELISA

Conjugate

Unconjugated

Cat No : 31087-1-AP

Print datasheet

Synonyms

Cxcl15, lungkine, Scyb15, weche

验证数据展示

  • IP experiment of mouse lung using 31087-1-AP

    IP experiment of mouse lung using 31087-1-AP

    IP result of anti-Cxcl15 (IP:31087-1-AP, 4ug; Detection:31087-1-AP 1:500) with mouse lung tissue lysate 1120 ug.

  • IHC staining of mouse lung using 31087-1-AP

    IHC staining of mouse lung using 31087-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31087-1-AP (Cxcl15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 31087-1-AP

    IHC staining of mouse lung using 31087-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31087-1-AP (Cxcl15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IP detected inmouse lung tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31087-1-AP targets Cxcl15 in IP, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Applications IHC, IP, ELISA
Tested Reactivity mouse
Immunogen Cxcl15 fusion protein Ag33964 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-X-C motif) ligand 15
Synonyms Cxcl15, lungkine, Scyb15, weche
Calculated Molecular Weight19 kDa
Observed Molecular Weight19 kDa
GenBank Accession NumberBC061138
Gene Symbol Cxcl15
Gene ID (NCBI) 20309
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9WVL7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cxcl15, also known as lungkine or WECHE, is a small cytokine belonging to the CXC chemokine family that has been described in the mouse. High levels of Cxcl15 mRNA were specifically detected in the lung and at lower levels in fetal lung tissue by Northern blot and in situ hybridization, suggesting a potential role for this chemokine during lung development. Moreover, the Cxcl15 protein is secreted into the airway spaces and induces the in vitro and in vivo migration of neutrophils, suggesting that it is involved in lung-specific neutrophil trafficking.

实验方案

Product Specific Protocols
IHC protocol for Cxcl15 antibody 31087-1-APDownload protocol
IP protocol for Cxcl15 antibody 31087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols