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验证数据展示

  • WB analysis using 31087-1-AP

    WB analysis using 31087-1-AP

    Mouse lung tissue were subjected to SDS PAGE followed by western blot with 31087-1-AP (CXL15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 31087-1-AP

    IP experiment of mouse lung using 31087-1-AP

    IP result of anti-Cxcl15 (IP:31087-1-AP, 4ug; Detection:31087-1-AP 1:500) with mouse lung tissue lysate 1120 ug.

  • IHC staining of mouse lung using 31087-1-AP

    IHC staining of mouse lung using 31087-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31087-1-AP (Cxcl15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 31087-1-AP

    IHC staining of mouse lung using 31087-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31087-1-AP (Cxcl15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CXL15 Polyclonal antibody

CXL15 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 31087-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IP, ELISA

别名

Cxcl15, chemokine (C X C motif) ligand 15, C-X-C motif chemokine 15, Cxcl-15, Scyb15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31087-1-AP targets CXL15 in WB, IHC, IP, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 mouse
免疫原 CXL15 fusion protein Ag33964 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-X-C motif) ligand 15
别名 Cxcl15, chemokine (C X C motif) ligand 15, C-X-C motif chemokine 15, Cxcl-15, Scyb15
计算分子量19 kDa
观测分子量19 kDa
GenBank蛋白编号BC061138
基因名称 Cxcl15
Gene ID (NCBI) 20309
RRIDAB_3669847
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9WVL7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cxcl15, also known as lungkine or WECHE, is a small cytokine belonging to the CXC chemokine family that has been described in the mouse. High levels of Cxcl15 mRNA were specifically detected in the lung and at lower levels in fetal lung tissue by Northern blot and in situ hybridization, suggesting a potential role for this chemokine during lung development. Moreover, the Cxcl15 protein is secreted into the airway spaces and induces the in vitro and in vivo migration of neutrophils, suggesting that it is involved in lung-specific neutrophil trafficking.

实验方案

Product Specific Protocols
WB protocol for CXL15 antibody 31087-1-APDownload protocol
IHC protocol for CXL15 antibody 31087-1-APDownload protocol
IP protocol for CXL15 antibody 31087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols