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验证数据展示

  • WB analysis using 12748-1-AP (same clone as 12748-1-PBS)

    WB analysis using 12748-1-AP (same clone as 12748-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 12748-1-AP (Complement factor H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12748-1-AP (same clone as 12748-1-PBS)

    IF Staining of HepG2 using 12748-1-AP (same clone as 12748-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Complement factor H antibody (12748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

  • IP experiment of human plasma using 12748-1-AP (same clone as 12748-1-PBS)

    IP experiment of human plasma using 12748-1-AP (same clone as 12748-1-PBS)

    IP result of anti-Complement factor H (IP:12748-1-AP, 4ug; Detection:12748-1-AP 1:1000) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 12748-1-AP (same clone as 12748-1-PBS)

    IHC staining of human liver cancer using 12748-1-AP (same clone as 12748-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CFH antibody included
  • IHC staining of human liver cancer using 12748-1-AP (same clone as 12748-1-PBS)

    IHC staining of human liver cancer using 12748-1-AP (same clone as 12748-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CFH antibody included
  • IF Staining of human liver cancer using 12748-1-AP (same clone as 12748-1-PBS)

    IF Staining of human liver cancer using 12748-1-AP (same clone as 12748-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Complement factor H antibody (12748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 12748-1-AP (same clone as 12748-1-PBS)

    IHC staining of human colon using 12748-1-AP (same clone as 12748-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CFH antibody included
  • IHC staining of human colon using 12748-1-AP (same clone as 12748-1-PBS)

    IHC staining of human colon using 12748-1-AP (same clone as 12748-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12748-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CFH antibody included

Complement factor H Polyclonal antibody, PBS Only

Complement factor H Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 12748-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CFH Ready-To-Use IHC Kit. CFH primary antibody included.

产品信息

12748-1-PBS targets Complement factor H in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3482

Product name: Recombinant human CFH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-349 aa of BC037285

Sequence: LPPRRNTEILTGSWSDQTYPEGTQAIYKCRPGYRSLGNVIMVCRKGEWVALNPLRKCQKRPCGHPGDTPFGTFTLTGGNVFEYGVKAVYTCNEGYQLLGEINYRECDTDGWTNDIPICEVVKCLPVTAPENGKIVSSAMEPDREYHFGQAVRFVCNSGYKIEGDEEMHCSDDGFWSKEKPKCVEISCKSPDVINGSPISQKIIYKENERFQYKCNMGYEYSERGDAVCTESGWRPLPSCEEKSCDNPYIPNGDYSPLRIKHRTGDEITYQCRNGFYPATRGNTAKCTSTGWIPAPRCTLKPCDYPDIKHGGLYHENMRRPYFPVAV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement factor H
别名
计算分子量 1231 aa, 139 kDa
观测分子量 140-150 kDa
GenBank蛋白编号BC037285
基因名称 Complement factor H
Gene ID (NCBI) 3075
RRIDAB_10642946
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP08603
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Complement factor H (CFH) is an abundant plasma glycoprotein that regulates the function of the alternative complement pathway in the fluid phase and on cellular surfaces (PMID: 2963625). Factor H binds to C3b, accelerates the decay of the alternative pathway C3-convertase (C3bBb), and acts as a cofactor for the factor I-mediated proteolytic inactivation of C3b (PMID: 15163532). It plays a critical role in the homeostasis of the complement system in plasma and in the protection of bystander host cells and tissues from damage by complement activation. Abnormalities in factor H have been associated with renal disease (PMID: 18190458).

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