验证数据展示
经过测试的应用
| Positive WB detected in | NCCIT cells, HeLa cells, A549 cells, pig colon tissue, pig rectum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68198-1-Ig targets CYP2S1 in WB, ELISA applications and shows reactivity with Human, Pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Pig |
| 免疫原 | CYP2S1 fusion protein Ag4605 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome P450, family 2, subfamily S, polypeptide 1 |
| 别名 | CYP2S1, CYPIIS1, Cytochrome P450 2S1 |
| 计算分子量 | 504 aa, 56 kDa |
| 观测分子量 | 50-56 kDa |
| GenBank蛋白编号 | BC033691 |
| 基因名称 | CYP2S1 |
| Gene ID (NCBI) | 29785 |
| RRID | AB_2935287 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96SQ9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The cytochrome P450 (CYP) family of isoenzymes are involved in the metabolism of diverse endogenous compounds and detoxification of exogenous agents. Cytochrome P450 2S1 (CYP2S1) is one 'orphan' CYP, and is particularly expressed in epithe-lial cells of tissues which are exposed to the environment such as the skin, respiratory, gastrointes-tinal, and urinary tracts. CYP2S1 is associated primarily with the synthesis and metabo-lism of lipids including prostaglandins (PGE2) and retinoids. In addition, CYP2S1 expression is associated with patient survival in breast cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP2S1 antibody 68198-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |