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验证数据展示

  • WB analysis of K-562 using 14840-1-AP

    WB analysis of K-562 using 14840-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14840-1-AP (CXCR7-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 14840-1-AP

    IP experiment of K-562 using 14840-1-AP

    IP result of anti-CXCR7-Specific (IP:14840-1-AP, 4ug; Detection:14840-1-AP 1:500) with K-562 cells lysate 3200ug.

  • IHC staining of mouse brain using 14840-1-AP

    IHC staining of mouse brain using 14840-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 14840-1-AP

    IHC staining of mouse brain using 14840-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CXCR7-Specific Polyclonal antibody

CXCR7-Specific Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14840-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, C6 cells, mouse brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14840-1-AP targets CXCR7-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-X-C motif) receptor 7
别名
计算分子量 41 kDa
观测分子量 50 kDa
GenBank蛋白编号NM_020311
基因名称 CXCR7
Gene ID (NCBI) 57007
RRIDAB_10646437
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25106
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor progression. This antibody is specific to CXCR7.

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