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验证数据展示

  • WB analysis of K-562 using 14840-1-AP

    WB analysis of K-562 using 14840-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14840-1-AP (CXCR7-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 14840-1-AP

    IP experiment of K-562 using 14840-1-AP

    IP result of anti-CXCR7-Specific (IP:14840-1-AP, 4ug; Detection:14840-1-AP 1:500) with K-562 cells lysate 3200ug.

  • IHC staining of mouse brain using 14840-1-AP

    IHC staining of mouse brain using 14840-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 14840-1-AP

    IHC staining of mouse brain using 14840-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14840-1-AP (CXCR7-Specific Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CXCR7-Specific Polyclonal antibody

CXCR7-Specific Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14840-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, C6 cells, mouse brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14840-1-AP targets CXCR7-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-X-C motif) receptor 7
别名
计算分子量 41 kDa
观测分子量 50 kDa
GenBank蛋白编号NM_020311
基因名称 CXCR7
Gene ID (NCBI) 57007
RRIDAB_10646437
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25106
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor progression. This antibody is specific to CXCR7.

实验方案

Product Specific Protocols
WB protocol for CXCR7-Specific antibody 14840-1-APDownload protocol
IHC protocol for CXCR7-Specific antibody 14840-1-APDownload protocol
IP protocol for CXCR7-Specific antibody 14840-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols