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验证数据展示

  • IHC staining of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

    IHC staining of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17532-1-AP (CTDSPL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTDSPL2 antibody included
  • WB analysis of MCF-7 using 17532-1-AP (same clone as 17532-1-PBS)

    WB analysis of MCF-7 using 17532-1-AP (same clone as 17532-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

    IHC staining of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17532-1-AP (CTDSPL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTDSPL2 antibody included
  • IP experiment of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

    IP experiment of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

    IP result of anti-CTDSPL2 (IP:17532-1-AP, 4ug; Detection:17532-1-AP 1:500) with mouse testis tissue lysate 4400ug. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 17532-1-AP (same clone as 17532-1-PBS)

    IHC staining of human testis using 17532-1-AP (same clone as 17532-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17532-1-AP (CTDSPL2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CTDSPL2 antibody included
  • IF Staining of HeLa using 17532-1-AP (same clone as 17532-1-PBS)

    IF Staining of HeLa using 17532-1-AP (same clone as 17532-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CTDSPL2 antibody (17532-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

CTDSPL2 Polyclonal antibody, PBS Only

CTDSPL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 17532-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

CTD small phosphatase-like protein 2, CTDSP like 2, CTDSP-like 2, EC:3.1.3.-, HSPC058

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® CTDSPL2 Ready-To-Use IHC Kit. CTDSPL2 primary antibody included.

产品信息

17532-1-PBS targets CTDSPL2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11581

Product name: Recombinant human CTDSPL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 114-466 aa of BC035744

Sequence: EMTNQHVKQNGKLEDNPSSGSPPRTTLLGTIFSPVFNFFSPANKNGTSGSDSPGQAVEAEEIVKQLDMEQVDEITTSTTTSTNGAAYSNQAVQVRPSLNNGLEEAEETVNRDIPPLTAPVTPDSGYSSAHAEATYEEDWEVFDPYYFIKHVPPLTEEQLNRKPALPLKTRSTPEFSLVLDLDETLVHCSLNELEDAALTFPVLFQDVIYQVYVRLRPFFREFLERMSQMYEIILFTASKKVYADKLLNILDPKKQLVRHRLFREHCVCVQGNYIKDLNILGRDLSKTIIIDNSPQAFAYQLSNGIPIESWFMDKNDNELLKLIPFLEKLVELNEDVRPHIRDRFRLHDLLPPD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 2
别名 CTD small phosphatase-like protein 2, CTDSP like 2, CTDSP-like 2, EC:3.1.3.-, HSPC058
计算分子量 466 aa, 53 kDa
观测分子量 67 kDa
GenBank蛋白编号BC035744
基因名称 CTDSPL2
Gene ID (NCBI) 51496
RRIDAB_2087204
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ05D32
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTD small phosphatase-like protein 2 (CTDSPL2) is also named as HSPC058 and HSPC129, belongs to the CTDSPL2 family. It is a putative RNA-polymerase II carboxy-terminal domain (CTD) phosphatase, whose specific CTD phosphatase activity was verified in vitro, and may regulate the dynamic phosphorylation of RNAP II CTD.( PMID: 17487459) It can obviously improve the expression of ε- and γ-globin genes in K562 cells and CD34+ cells derived from UCB.( PMID: 20932329) This antibody is a rabbit polyclonal antibody raised against a region of human CTDSPL2.

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