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验证数据展示

  • WB analysis using 30498-1-AP

    WB analysis using 30498-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30498-1-AP (CSN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 30498-1-AP

    IHC staining of human breast cancer using 30498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast hyperplasia using 30498-1-AP

    IHC staining of human breast hyperplasia using 30498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast hyperplasia using 30498-1-AP

    IHC staining of human breast hyperplasia using 30498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 30498-1-AP

    WB analysis using 30498-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 30498-1-AP (CSN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

CSN2 Polyclonal antibody

CSN2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30498-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

Beta casein, CASB, casein beta, CSN2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HT-29 cells, NIH/3T3 cells, mouse liver tissue, mouse testis tissue, DU 145 cells, rat liver tissue
Positive IHC detected inhuman breast hyperplasia tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30498-1-AP targets CSN2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag27943

Product name: Recombinant human CSN2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 18-226 aa of BC096194

Sequence: TIESLSSSEESITEYKQKVEKVKHEDQQQGEDEHQDKIYPSFQPQPLIYPFVEPIPYGFLPQNILPLAQPAVVLPVPQPEIMEVPKAKDTVYTKGRVMPVLKSPTIPFFDPQIPKLTDLENLHLPLPLLQPLMQQVPQPIPQTLALPPQPLWSVPQPKVLPIPQQVVPYPQRAVPVQALLLNQELLLNPTHQIYPVTQPLAPVHNPISV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 casein beta
别名 Beta casein, CASB, casein beta, CSN2
观测分子量 60 kDa
GenBank蛋白编号BC096194
基因名称 CSN2
Gene ID (NCBI) 1447
RRIDAB_3086338
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05814
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSN2 is also named as Beta-casein and CASB.The β-casein is the most abundant protein in camel milk, and is one of the most important milk protein fractions  (PMID: 24973699).

实验方案

Product Specific Protocols
IHC protocol for CSN2 antibody 30498-1-APDownload protocol
WB protocol for CSN2 antibody 30498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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