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验证数据展示

  • WB analysis using 30498-1-AP

    WB analysis using 30498-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30498-1-AP (CSN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 30498-1-AP

    IHC staining of human breast cancer using 30498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast hyperplasia using 30498-1-AP

    IHC staining of human breast hyperplasia using 30498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast hyperplasia using 30498-1-AP

    IHC staining of human breast hyperplasia using 30498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 30498-1-AP

    WB analysis using 30498-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 30498-1-AP (CSN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

CSN2 Polyclonal antibody

CSN2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30498-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

Beta casein, CASB, casein beta, CSN2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HT-29 cells, NIH/3T3 cells, mouse liver tissue, mouse testis tissue, DU 145 cells, rat liver tissue
Positive IHC detected inhuman breast hyperplasia tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30498-1-AP targets CSN2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 CSN2 fusion protein Ag27943 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 casein beta
别名 Beta casein, CASB, casein beta, CSN2
观测分子量 60 kDa
GenBank蛋白编号BC096194
基因名称 CSN2
Gene ID (NCBI) 1447
RRIDAB_3086338
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05814
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSN2 is also named as Beta-casein and CASB.The β-casein is the most abundant protein in camel milk, and is one of the most important milk protein fractions  (PMID: 24973699).

实验方案

Product Specific Protocols
WB protocol for CSN2 antibody 30498-1-APDownload protocol
IHC protocol for CSN2 antibody 30498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols