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CSN2 Polyclonal antibody

CSN2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 30498-1-AP

Print datasheet

Synonyms

Beta casein, CASB, casein beta, CSN2

验证数据展示

  • WB analysis using 30498-1-AP

    WB analysis using 30498-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30498-1-AP (CSN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 30498-1-AP

    IHC staining of human breast cancer using 30498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast hyperplasia using 30498-1-AP

    IHC staining of human breast hyperplasia using 30498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast hyperplasia using 30498-1-AP

    IHC staining of human breast hyperplasia using 30498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 30498-1-AP (CSN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 30498-1-AP

    WB analysis using 30498-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 30498-1-AP (CSN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HT-29 cells, NIH/3T3 cells, mouse liver tissue, mouse testis tissue, DU 145 cells, rat liver tissue
Positive IHC detected inhuman breast hyperplasia tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30498-1-AP targets CSN2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen CSN2 fusion protein Ag27943 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name casein beta
Synonyms Beta casein, CASB, casein beta, CSN2
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC096194
Gene Symbol CSN2
Gene ID (NCBI) 1447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05814
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSN2 is also named as Beta-casein and CASB.The β-casein is the most abundant protein in camel milk, and is one of the most important milk protein fractions  (PMID: 24973699).

实验方案

Product Specific Protocols
WB protocol for CSN2 antibody 30498-1-APDownload protocol
IHC protocol for CSN2 antibody 30498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols