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验证数据展示

  • WB analysis using 29497-1-AP

    WB analysis using 29497-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 29497-1-AP (CRMP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29497-1-AP

    IHC staining of mouse brain using 29497-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29497-1-AP (CRMP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CRMP1 Polyclonal antibody

CRMP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29497-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Mouse, Rat

应用

WB, IHC, ELISA

别名

CRMP 1, CRMP1, DPYSL1, DRP 1, DRP1, ULIP 3, ULIP3, Unc 33 like phosphoprotein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29497-1-AP targets CRMP1 in WB, IHC, ELISA applications and shows reactivity with Mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Mouse, Rat
免疫原 CRMP1 fusion protein Ag31559 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collapsin response mediator protein 1
别名 CRMP 1, CRMP1, DPYSL1, DRP 1, DRP1, ULIP 3, ULIP3, Unc 33 like phosphoprotein 3
计算分子量62KD
观测分子量62 kDa
GenBank蛋白编号NM_001014809
基因名称 CRMP1
Gene ID (NCBI) 1400
RRIDAB_2918317
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14194
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRMP1 (Collapsin response mediator protein 1) is also named as DPYSL1, ULIP3, DRP-1 and belongs to the collapsin response mediator protein family, which are a family of five cytoplasmic proteins predominantly expressed in the developing nervous system. CRMP1, the predicted 62-kDa protein and its 75-kDa isoform corresponding to its N-terminal splice variant already described for embryonic, neonatal, and adult brain of the rat are evidenced in the cortex of adult mouse (PMID:15834957). This protein is involved in invasion, and aggressiveness of prolactin pituitary tumors.

实验方案

Product Specific Protocols
WB protocol for CRMP1 antibody 29497-1-APDownload protocol
IHC protocol for CRMP1 antibody 29497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols