验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
12311-1-AP targets CHD2 in WB, IHC, IP, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | CHD2 fusion protein Ag2960 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromodomain helicase DNA binding protein 2 |
| 别名 | ATP dependent helicase CHD2, CHD 2, CHD2 |
| 计算分子量 | 1828 aa, 211 kDa |
| 观测分子量 | 95 kDa |
| GenBank蛋白编号 | BC020810 |
| 基因名称 | CHD2 |
| Gene ID (NCBI) | 1106 |
| RRID | AB_2079925 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14647 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Chromodomain-helicase-DNA-binding protein 2, also named as ATP-dependent helicase CHD2, CHD2. It is a sequence-selective DNA-binding protein. Molecular mass of CHD2 is 211kD (201kd for isoform 2, 57kd for isoform 3). CHD2 is confirmed around 95 kDa at the undifferentiated stage. This clearly indicated that CHD2 was involved in specific gene expression at this stage.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHD2 antibody 12311-1-AP | Download protocol |
| IHC protocol for CHD2 antibody 12311-1-AP | Download protocol |
| IP protocol for CHD2 antibody 12311-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination. | ||
PeerJ Identification and validation of immune related core transcription factors GTF2I in NAFLD. | ||