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验证数据展示

  • WB analysis using 32395-1-AP

    WB analysis using 32395-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32395-1-AP (CEACAM-1/CD66a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 32395-1-AP

    IHC staining of mouse spleen using 32395-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32395-1-AP (CEACAM-1/CD66a antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 32395-1-AP

    IHC staining of mouse spleen using 32395-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32395-1-AP (CEACAM-1/CD66a antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse spleen using 32395-1-AP

    IF Staining of mouse spleen using 32395-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CEACAM-1/CD66a antibody (32395-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CEACAM-1/CD66a Polyclonal antibody

CEACAM-1/CD66a Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 32395-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF-P, ELISA

别名

Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein D, Biliary glycoprotein 1, BGP-1, Bgp1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse colon tissue, mouse liver tissue, mouse small intestine
Positive IF-P detected inmouse spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32395-1-AP targets CEACAM-1/CD66a in WB, IF-P, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carcinoembryonic antigen-related cell adhesion molecule 1
别名 Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein D, Biliary glycoprotein 1, BGP-1, Bgp1
计算分子量57 kDa
观测分子量100-120 kDa
基因名称 Ceacam1
Gene ID (NCBI) 26365
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP31809-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

实验方案

Product Specific Protocols
WB protocol for CEACAM-1/CD66a antibody 32395-1-APDownload protocol
IF protocol for CEACAM-1/CD66a antibody 32395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols