1x10^6 untreated or LPS treated mouse splenic leukocytes were surface stained with 0.25 ug Anti-Mouse CD70 Rabbit Recombinant Antibody (98212-1-RR, Clone: 241553F2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

1x10^6 LPS treated mouse splenic leukocytes were surface stained with 0.25 ug Anti-Mouse CD70 Rabbit Recombinant Antibody (98212-1-RR, Clone: 241553F2) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 LPS treated Mouse splenic leucocytes were surface stained with 0.25 ug Anti-Mouse CD70 Rabbit Recombinant Antibody (98212-1-RR, Clone: 241553F2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

Biolayer interferometry (BLl) kinetic assays of 98212-1-RR against Mouse CD70 were performed. The affinity constant is 37.8 pM.