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验证数据展示

  • FC experiment of rabbit splenocytes using 98306-6-MR

    FC experiment of rabbit splenocytes using 98306-6-MR

    1x10^6 rabbit splenocytes were surface stained with 0.25 ug Anti-Rabbit CD38 Mouse RecAb (98306-6-MR, Clone: 250406C8) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Nano-Secondary® anti-mouse IgG2a(recombinant VHH). Cells were co-stained with CoraLite® Plus 488 Anti-Rabbit CD3. Cells were not fixed.

  • FC experiment of rabbit splenocytes using 98306-6-MR

    FC experiment of rabbit splenocytes using 98306-6-MR

    1x10^6 rabbit splenocytes were surface stained with 0.25 ug Anti-Rabbit CD38 Mouse RecAb (98306-6-MR, Clone: 250406C8) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Nano-Secondary® anti-mouse IgG2a(recombinant VHH). Cells were co-stained with CoraLite® Plus 488 Anti-Rabbit CD3. Cells were not fixed.

  • Affinity of 98306-6-MR

    Affinity of 98306-6-MR

    Biolayer interferometry (BLl) kinetic assays of 98306-6-MR against Rabbit CD38 were performed. The affinity constant is 3.14 nM.

Anti-Rabbit CD38 Mouse Recombinant Antibody

CD38 Uni-rAb® Recombinant Antibody for FC
Cat No. 98306-6-MR

产品说明书

CloneNo. 250406C8

宿主/亚型

Mouse / IgG2a

种属反应性

rabbit

应用

FC

别名

ADP ribosyl cyclase/cyclic ADP ribose hydrolase, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, CD38 antigen, Cyclic ADP ribose hydrolase 1

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inrabbit splenocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98306-6-MR targets CD38 in FC applications and shows reactivity with rabbit samples.

经测试应用 FC Application Description
经测试反应性 rabbit
免疫原

CatNo: Eg2484

Product name: recombinant rabbit CD38 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 10007021 of

Sequence: /

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 CD38
别名 ADP ribosyl cyclase/cyclic ADP ribose hydrolase, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, CD38 antigen, Cyclic ADP ribose hydrolase 1
基因名称 CD38
Gene ID (NCBI) 100009409
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9MZ03
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

实验方案

Product Specific Protocols
FC protocol for CD38 antibody 98306-6-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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