×
Use Able AI chat for product recommendations

验证数据展示

  • FC experiment of human peripheral blood lymphocytes using 98230-2-RR

    FC experiment of human peripheral blood lymphocytes using 98230-2-RR

    1x10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug Anti-Human CD367/CLEC4A Rabbit RecAb (98230-2-RR, Clone: 241854D7) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human peripheral blood lymphocytes using 98230-2-RR

    FC experiment of human peripheral blood lymphocytes using 98230-2-RR

    1x10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug Anti-Human CD367/CLEC4A Rabbit RecAb (98230-2-RR, Clone: 241854D7) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • Affinity of 98230-2-RR

    Affinity of 98230-2-RR

    Biolayer interferometry (BLl) kinetic assays of 98230-2-RR against Human CD367/CLEC4A were performed. The affinity constant is 0.70 nM.

Anti-Human CD367/CLEC4A Rabbit Recombinant Antibody

CD367/CLEC4A Uni-rAb® Recombinant Antibody for FC
Cat No. 98230-2-RR

产品说明书

CloneNo. 241854D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

CLEC4A, CD367, CLECSF6, DCIR, DDB27

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC detected inhuman peripheral blood lymphocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98230-2-RR targets CD367/CLEC4A in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg1350

Product name: Recombinant Human DCIR/CLEC4A protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 70-237 aa of NM_016184.4

Sequence: QKYSQLLEKKTTKELVHTTLECVKKNMPVEETAWSCCPKNWKSFSSNCYFISTESASWQDSEKDCARMEAHLLVINTQEEQDFIFQNLQEESAYFVGLSDPEGQRHWQWVDQTPYNESSTFWHPREPSDPNERCVVLNFRKSPKRWGWNDVNCLGPQRSVCEMMKIHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 C-type lectin domain family 4, member A
别名 CLEC4A, CD367, CLECSF6, DCIR, DDB27
计算分子量28 kDa
GenBank蛋白编号NM_016184.4
基因名称 CLEC4A
Gene ID (NCBI) 50856
RRIDAB_3745897
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UMR7-1
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD367 (CLEC4A/DCIR) is a C-type lectin receptor primarily expressed on antigen-presenting cells. CD367 features a characteristic C-type lectin domain (CRD) in its extracellular region that recognizes carbohydrate ligands in a calcium-dependent manner and exhibits a restricted hematopoietic expression profile particularly useful for dendritic cell (DC) subset identification.

实验方案

Product Specific Protocols
FC protocol for CD367/CLEC4A antibody 98230-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...