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验证数据展示

  • FC experiment of human PBMCs using 65536-1-PBS

    FC experiment of human PBMCs using 65536-1-PBS

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (65536-1-PBS, Clone: UCHT1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.25 ug Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (blue). Cells were not fixed.

  • FC experiment of human PBMCs using 65536-1-MR (same clone as 65536-1-PBS)

    FC experiment of human PBMCs using 65536-1-MR (same clone as 65536-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (65536-1-MR, Clone:UCHT1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1) (blue). Cells were not fixed. This data was developed using the same antibody clone with 65536-1-PBS in a different storage buffer formulation.

Anti-Human CD3 (UCHT1) Mouse IgG1 Recombinant Antibody, PBS Only

CD3 Recombinant Antibody for FC
Cat No. 65536-1-PBS

产品说明书

CloneNo. UCHT1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC

别名

CD3E, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T3E

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65536-1-PBS targets CD3 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Recombinant
产品类型 Antibody
全称 CD3e molecule, epsilon (CD3-TCR complex)
别名 CD3E, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T3E
计算分子量 207 aa, 23 kDa
GenBank蛋白编号BC049847
基因名称 CD3
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

实验方案

Product Specific Protocols
FC protocol for CD3 antibody 65536-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols