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验证数据展示

  • IHC staining of mouse spleen using 31263-1-AP

    IHC staining of mouse spleen using 31263-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31263-1-AP (CD19 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 31263-1-AP

    IHC staining of mouse spleen using 31263-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31263-1-AP (CD19 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD19 Polyclonal antibody

CD19 Polyclonal Antibody for IHC, ELISA
Cat No. 31263-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

IHC, ELISA

别名

CD19 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse spleen tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31263-1-AP targets CD19 in IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 CD19 fusion protein Eg0907 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD19 antigen
别名 CD19 antigen
计算分子量60 kDa
GenBank蛋白编号NM_009844.2
基因名称 Cd19
Gene ID (NCBI) 12478
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP25918
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

实验方案

Product Specific Protocols
IHC protocol for CD19 antibody 31263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols