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验证数据展示

  • WB analysis using 32435-1-AP

    WB analysis using 32435-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32435-1-AP (CD107a / LAMP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 32435-1-AP

    WB analysis using 32435-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32435-1-AP (CD107a / LAMP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 32435-1-AP

    IF Staining of NIH/3T3 using 32435-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Lamp1 antibody (32435-1-AP) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

CD107a / LAMP1 Polyclonal antibody

CD107a / LAMP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32435-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF/ICC, ELISA

别名

CD107a, Lamp1, Lamp 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, C6 cells, HSC-T6 cells, rat kidney tissue, RAW 264.7 cells
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32435-1-AP targets CD107a / LAMP1 in WB, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Ag34623

Product name: Recombinant mouse Lamp1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-190 aa of NM_001317353

Sequence: MAAPGARRPLLLLLLAGLAHGASALFEVKNNGTTCIMASFSASFLTTYETANGSQIVNISLPASAEVLKNGSSCGKENVSDPSLTITFGRGYLLTLNFTKNTTRYSVQHMYFTYNLSDTEHFPNAISKEIYTMDSTTDIKADINKAYRCVSDIRVYMKNVTVVLRDATIQAYLSSGNFSKEETHCTQDGP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lysosomal-associated membrane protein 1
别名 CD107a, Lamp1, Lamp 1
计算分子量 44 kDa
观测分子量 90-120 kDa
GenBank蛋白编号NM_001317353
基因名称 CD107a
Gene ID (NCBI) 16783
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP11438
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

实验方案

Product Specific Protocols
IF protocol for CD107a / LAMP1 antibody 32435-1-APDownload protocol
WB protocol for CD107a / LAMP1 antibody 32435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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