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验证数据展示

  • FC experiment of NR8383 using 98770-4-RR

    FC experiment of NR8383 using 98770-4-RR

    1x10^6 untreated or LPS-treated NR8383 cells were intracellularly stained with 0.25 ug Anti-Rat CCL4/MIP-1 beta Rabbit RecAb (98770-4-RR, Clone: 251973F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • FC experiment of NR8383 using 98770-4-RR

    FC experiment of NR8383 using 98770-4-RR

    1x10^6 LPS treated NR8383 cells were intracellularly stained with 0.25 ug Anti-Rat CCL4/MIP-1 beta Rabbit RecAb (98770-4-RR, Clone: 251973F1)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated NR8383 were surface stained with 0.25 ug Anti-Rat CCL4/MIP-1 beta Rabbit RecAb (98770-4-RR, Clone: 251973F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Anti-Rat CCL4/MIP-1 beta Rabbit Recombinant Antibody

CCL4/MIP-1 beta Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98770-4-RR

产品说明书

CloneNo. 251973F1

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

FC (Intra)

别名

C-C motif chemokine 4, Ccl4, CCL-4, Macrophage inflammatory protein 1-beta, MIP-1 beta

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inLPS treated NR8383 cells, Transfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98770-4-RR targets CCL4/MIP-1 beta in FC (Intra) applications and shows reactivity with rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 rat
免疫原

CatNo: Eg3200

Product name: recombinant rat CCL4/MIP-1 beta protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 24-92 aa of NM_053858.1

Sequence: APIGSDPPTSCCFSYTSRKIHRNFVMDYYETSSLCSQPAVVFLTKKGRQICADPSEPWVNEYVNDLELN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-C motif) ligand 4
别名 C-C motif chemokine 4, Ccl4, CCL-4, Macrophage inflammatory protein 1-beta, MIP-1 beta
计算分子量10 kDa
GenBank蛋白编号NM_053858.1
基因名称 Ccl4
Gene ID (NCBI) 116637
RRIDAB_3746336
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP50230
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

CCL4, also known as Macrophage inflammatory protein-1β (MIP-1β), is a CC chemokine with specificity for CCR5 receptors, and is one of the major HIV-suppressive factors produced by CD8+ T-cells. MIP-1β is an acidic protein composed of 69 amino acids that is produced by many cells, particularly macrophages, dendritic cells, and lymphocytes. MIP-1β is responsible for the activation of PMN and is involved in acute neutrophilic inflammation. Recombinant MIP-1β induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).

实验方案

Product Specific Protocols
FC protocol for CCL4/MIP-1 beta antibody 98770-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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