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验证数据展示

  • WB analysis using 32190-1-AP

    WB analysis using 32190-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32190-1-AP (CCL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 32190-1-AP

    IF Staining of THP-1 using 32190-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PMA, LPS and Brefeldin A treated THP-1 cells using CCL3 antibody (32190-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CCL3 Polyclonal antibody

CCL3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32190-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

MIP-1-alpha, Mip1a, Macrophage inflammatory protein 1-alpha, L2G25B, Heparin-binding chemotaxis protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLPS and Brefeldin A treated RAW 264.7 cells
Positive IF/ICC detected inPMA, LPS and Brefeldin A treated THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32190-1-AP targets CCL3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-C motif) ligand 3
别名 MIP-1-alpha, Mip1a, Macrophage inflammatory protein 1-alpha, L2G25B, Heparin-binding chemotaxis protein
计算分子量10kd
观测分子量11 kDa
GenBank蛋白编号NM_011337
基因名称 Ccl3
Gene ID (NCBI) 20302
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP10855
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCL3 antibody 32190-1-APDownload protocol
IF protocol for CCL3 antibody 32190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols