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验证数据展示

  • WB analysis using 32190-1-AP

    WB analysis using 32190-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32190-1-AP (CCL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 32190-1-AP

    IF Staining of THP-1 using 32190-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PMA, LPS and Brefeldin A treated THP-1 cells using CCL3 antibody (32190-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CCL3/MIP-1 alpha Polyclonal antibody

CCL3/MIP-1 alpha Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32190-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

C-C motif chemokine 3, Ccl3, Heparin-binding chemotaxis protein, L2G25B, Macrophage inflammatory protein 1-alpha

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLPS and Brefeldin A treated RAW 264.7 cells
Positive IF/ICC detected inPMA, LPS and Brefeldin A treated THP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32190-1-AP targets CCL3/MIP-1 alpha in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CCL3/MIP-1 alpha fusion protein Eg2170 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-C motif) ligand 3
别名 C-C motif chemokine 3, Ccl3, Heparin-binding chemotaxis protein, L2G25B, Macrophage inflammatory protein 1-alpha
计算分子量10kd
观测分子量11 kDa
GenBank蛋白编号NM_011337
基因名称 Ccl3
Gene ID (NCBI) 20302
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP10855
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Chemokine (C-C motif) ligand 3 (CCL3), also known as MIP-1α, belongs to the family of chemokines. CCL3 has been found in the central nervous system and its cognate receptors, CCR1 and CCR5, have been reported to be expressed by astrocytes, microglia and neurons. CCL3 and its receptors, CCR1 and CCR5, also contribute to the development of bone disease in multiple myeloma by supporting tumor growth and regulating osteoclast differentiation. CCL3 is also associated with the regulation of cell growth, angiogenesis, and metastasis of different tumors such as melanoma, renal cell carcinoma, and colorectal cancer. Moreover, CCL3 enhances cell migration and metastasis by up-regulating matrix metalloproteinase-2 (MMP)-2 expression in chondrosarcoma cells.

实验方案

Product Specific Protocols
WB protocol for CCL3/MIP-1 alpha antibody 32190-1-APDownload protocol
IF protocol for CCL3/MIP-1 alpha antibody 32190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols