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验证数据展示

  • WB analysis of HEK-293T using 86607-3-RR (same clone as 86607-3-PBS)

    WB analysis of HEK-293T using 86607-3-RR (same clone as 86607-3-PBS)

    Non-transfected and rat CCL20-transfected HEK-293T cells were subjected to SDS PAGE followed by western blot with 86607-3-RR (CCL20/MIP-3 alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86607-3-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 86607-3-RR (same clone as 86607-3-PBS)

    IHC staining of rat liver using 86607-3-RR (same clone as 86607-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 86607-3-RR (CCL20/MIP-3 alpha antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86607-3-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 86607-3-RR (same clone as 86607-3-PBS)

    IHC staining of rat liver using 86607-3-RR (same clone as 86607-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 86607-3-RR (CCL20/MIP-3 alpha antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86607-3-PBS in a different storage buffer formulation.

  • IF Staining of Transfected CHO-K1 using 86607-3-RR (same clone as 86607-3-PBS)

    IF Staining of Transfected CHO-K1 using 86607-3-RR (same clone as 86607-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Transfected CHO-K1 using CCL20/MIP-3 alpha antibody (86607-3-RR, Clone: 250116E12 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 86607-3-PBS in a different storage buffer formulation.

Rat CCL20/MIP-3 alpha Recombinant monoclonal antibody, PBS Only

CCL20/MIP-3 alpha Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86607-3-PBS

产品说明书

CloneNo. 250116E12

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Beta-chemokine exodus-1, CC chemokine LARC, CC chemokine ST38, C-C motif chemokine 20, CCL 20

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86607-3-PBS targets CCL20/MIP-3 alpha in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 rat
免疫原

CatNo: Eg3237

Product name: Recombinant Rat CCL20/MIP-3 alpha protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 27-96 aa of NM_019233.1

Sequence: SNFDCCLTYTKNVYHHARNFVGFTTQMADEACDINAIIFHLKSKRSVCADPKQIWVKRILHLLSLRTKKM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-C motif) ligand 20
别名 Beta-chemokine exodus-1, CC chemokine LARC, CC chemokine ST38, C-C motif chemokine 20, CCL 20
计算分子量11 kDa
GenBank蛋白编号NM_019233.1
基因名称 Ccl20
Gene ID (NCBI) 29538
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP97884
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.

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