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验证数据展示

  • WB analysis using 32080-1-AP

    WB analysis using 32080-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32080-1-AP (CCDC47 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 32080-1-AP

    IHC staining of mouse testis using 32080-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32080-1-AP (CCDC47 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 32080-1-AP

    IHC staining of mouse cerebellum using 32080-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32080-1-AP (CCDC47 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 32080-1-AP

    IHC staining of mouse cerebellum using 32080-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32080-1-AP (CCDC47 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CCDC47 Polyclonal antibody

CCDC47 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32080-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Calumin, Coiled-coil domain-containing protein 47, GK001, MSTP041, PAT complex subunit CCDC47

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, L02 cells, RT-4 cells, mouse heart tissue, mouse lung tissue, rat heart tissue
Positive IHC detected inmouse cerebellum tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32080-1-AP targets CCDC47 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CCDC47 fusion protein Ag36570 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 47
别名 Calumin, Coiled-coil domain-containing protein 47, GK001, MSTP041, PAT complex subunit CCDC47
计算分子量56 kDa
观测分子量56 kDa
GenBank蛋白编号BC008905
基因名称 CCDC47
Gene ID (NCBI) 57003
RRIDAB_3670185
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96A33
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coiled-coil domain containing 47 (CCDC47, also known as THNS, GK001, and MSTP041) is an endoplasmic reticulum (ER) transmembrane protein involved in calcium signaling through utilization of its calcium binding-acidic luminal domain. It also interacts with the ERAD (endoplasmic reticulum-associated degradation) complex and is involved in ER stress relief (PMID: 28974366). The expression level of CCDC47 is associated with diabetic cardiomyopathy (PMID: 30140426). Its variant would cause trichohepatoneurodevelopmental syndrome (PMID: 38524542).

实验方案

Product Specific Protocols
WB protocol for CCDC47 antibody 32080-1-APDownload protocol
IHC protocol for CCDC47 antibody 32080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols