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验证数据展示

  • WB analysis using 32080-1-AP

    WB analysis using 32080-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32080-1-AP (CCDC47 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 32080-1-AP

    IHC staining of mouse testis using 32080-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 32080-1-AP (CCDC47 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 32080-1-AP

    IHC staining of mouse cerebellum using 32080-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32080-1-AP (CCDC47 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 32080-1-AP

    IHC staining of mouse cerebellum using 32080-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32080-1-AP (CCDC47 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CCDC47 Polyclonal antibody

CCDC47 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32080-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Calumin, Coiled-coil domain-containing protein 47, GK001, MSTP041, PAT complex subunit CCDC47

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, L02 cells, RT-4 cells, mouse heart tissue, mouse lung tissue, rat heart tissue
Positive IHC detected inmouse cerebellum tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32080-1-AP targets CCDC47 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag36570

Product name: Recombinant human CCDC47 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 156-483 aa of BC008905

Sequence: GKNKNSRLAQAWFNTHRELLESNFTLVGDDGTNKEATSTGKLNQENEHIYNLWCSGRVCCEGMLIQLRFLKRQDLLNVLARMMRPVSDQVQIKVTMNDEDMDTYVFAVGTRKALVRLQKEMQDLSEFCSDKPKSGAKYGLPDSLAILSEMGEVTDGMMDTKMVHFLTHYADKIESVHFSDQFSGPKIMQEEGQPLKLPDTKRTLLFTFNVPGSGNTYPKDMEALLPLMNMVIYSIDKAKKFRLNREGKQKADKNRARVEENFLKLTHVQRQEAAQSRREEKKRAEKERIMNEEDPEKQRRLEEAALRREQKKLEKKQMKMKQIKVKAM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 47
别名 Calumin, Coiled-coil domain-containing protein 47, GK001, MSTP041, PAT complex subunit CCDC47
计算分子量56 kDa
观测分子量56 kDa
GenBank蛋白编号BC008905
基因名称 CCDC47
Gene ID (NCBI) 57003
RRIDAB_3670185
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96A33
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Coiled-coil domain containing 47 (CCDC47, also known as THNS, GK001, and MSTP041) is an endoplasmic reticulum (ER) transmembrane protein involved in calcium signaling through utilization of its calcium binding-acidic luminal domain. It also interacts with the ERAD (endoplasmic reticulum-associated degradation) complex and is involved in ER stress relief (PMID: 28974366). The expression level of CCDC47 is associated with diabetic cardiomyopathy (PMID: 30140426). Its variant would cause trichohepatoneurodevelopmental syndrome (PMID: 38524542).

实验方案

Product Specific Protocols
IHC protocol for CCDC47 antibody 32080-1-APDownload protocol
WB protocol for CCDC47 antibody 32080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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