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验证数据展示

  • WB analysis using 15619-1-AP (same clone as 15619-1-PBS)

    WB analysis using 15619-1-AP (same clone as 15619-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15619-1-AP (CBR3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15619-1-AP (same clone as 15619-1-PBS)

    IHC staining of human colon cancer using 15619-1-AP (same clone as 15619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15619-1-AP (CBR3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 15619-1-AP (same clone as 15619-1-PBS)

    IHC staining of human colon cancer using 15619-1-AP (same clone as 15619-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15619-1-AP (CBR3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 15619-1-AP (same clone as 15619-1-PBS)

    IF Staining of HeLa using 15619-1-AP (same clone as 15619-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15619-1-AP (CBR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15619-1-PBS in a different storage buffer formulation.

CBR3 Polyclonal antibody, PBS Only

CBR3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15619-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

15619-1-PBS targets CBR3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8039

Product name: Recombinant human CBR3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-277 aa of BC002812

Sequence: MSSCSRVALVTGANRGIGLAIARELCRQFSGDVVLTARDVARGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLNVLVNNAAVAFKSDDPMPFDIKAEMTLKTNFFATRNMCNELLPIMKPHGRVVNISSLQCLRAFENCSEDLQERFHSETLTEGDLVDLMKKFVEDTKNEVHEREGWPNSPYGVSKLGVTVLSRILARRLDEKRKADRILVNACCPGPVKTDMDGKDSIRTVEEGAETPVYLALLPPDATEPQGQLVHDKVVQNW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbonyl reductase 3
别名
计算分子量 31 kDa
观测分子量 31 kDa
GenBank蛋白编号BC002812
基因名称 CBR3
Gene ID (NCBI) 874
RRIDAB_2070862
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75828
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CBR3(Carbonyl reductase [NADPH] 3), which Belongs to the short-chain dehydrogenases/reductases (SDR) family, has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro). The gene encodes a 31 kDa protein that can catalyze the reduction of many endogenous and xenobiotic carbonyl compounds, including steroids and prostaglandins. The deduced 277-amino acid CBR3 protein is 84% similar to the CBR1 protein(PMID:9740676).

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