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验证数据展示

  • WB analysis of HeLa using 23821-1-AP

    WB analysis of HeLa using 23821-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23821-1-AP (CASP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 23821-1-AP

    IHC staining of human pancreas using 23821-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23821-1-AP (CASP9 Antibody) at dilution of 1:50.

  • IHC staining of human pancreas using 23821-1-AP

    IHC staining of human pancreas using 23821-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23821-1-AP (CASP9 Antibody) at dilution of 1:50.

  • IF Staining of HUVEC using 23821-1-AP

    IF Staining of HUVEC using 23821-1-AP

    Immunofluorescent analysis of HUVEC cells using 23821-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Caspase 9/p35/p10 Polyclonal antibody

Caspase 9/p35/p10 Polyclonal Antibody for WB, IF, IHC, ELISA
Cat No. 23821-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF, IHC, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells,
Positive IHC detected inhuman pancreas tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23821-1-AP targets Caspase 9/p35/p10 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原 Caspase 9/p35/p10 fusion protein Ag20813 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 caspase 9, apoptosis-related cysteine peptidase
别名
计算分子量 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC002452
基因名称 Caspase 9
Gene ID (NCBI) 842
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP55211
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. CASP9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade

发表文章

SpeciesApplicationTitle
mouseWB

J Cell Physiol

Alpha-Lipoic Acid Promotes Osteoblastic Formation in H2 O2 -Treated MC3T3-E1 Cells and Prevents Bone Loss in Ovariectomized Rats.

Authors - Chao Fu
View Article