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Caspase 9/p35/p10 Polyclonal antibody

Caspase 9/p35/p10 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 23821-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HeLa using 23821-1-AP

    WB analysis of HeLa using 23821-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23821-1-AP (CASP9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 23821-1-AP

    IHC staining of human pancreas using 23821-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23821-1-AP (CASP9 Antibody) at dilution of 1:50.

  • IHC staining of human pancreas using 23821-1-AP

    IHC staining of human pancreas using 23821-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23821-1-AP (CASP9 Antibody) at dilution of 1:50.

  • IF Staining of HUVEC using 23821-1-AP

    IF Staining of HUVEC using 23821-1-AP

    Immunofluorescent analysis of HUVEC cells using 23821-1-AP (Caspase 9/p35/p10 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inHeLa cells,
Positive IHC detected inhuman pancreas tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 23821-1-AP is Caspase 9/p35/p10 Fusion Protein expressed in E. coli.

Tested Applications IF, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen Caspase 9/p35/p10 fusion protein Ag20813 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caspase 9, apoptosis-related cysteine peptidase
Synonyms
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC002452
Gene Symbol CASP9
Gene ID (NCBI) 842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55211
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. CASP9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade

发表文章

SpeciesApplicationTitle
mouseWB

J Cell Physiol

Alpha-Lipoic Acid Promotes Osteoblastic Formation in H2 O2 -Treated MC3T3-E1 Cells and Prevents Bone Loss in Ovariectomized Rats.

Authors - Chao Fu
View Article