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验证数据展示

  • WB analysis of NIH/3T3 using 24001-1-AP

    WB analysis of NIH/3T3 using 24001-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24001-1-AP (C7orf63 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 24001-1-AP

    IHC staining of mouse testis using 24001-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24001-1-AP (C7orf63 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 24001-1-AP

    IHC staining of mouse testis using 24001-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24001-1-AP (C7orf63 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 24001-1-AP

    IF Staining of mouse testis using 24001-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using C7orf63 antibody (24001-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 24001-1-AP

    IF Staining of mouse testis using 24001-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using C7orf63 antibody (24001-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat testis using 24001-1-AP

    IF Staining of rat testis using 24001-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat testis tissue using C7orf63 antibody (24001-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C7orf63 Polyclonal antibody

C7orf63 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 24001-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

CFAP69, Cilia- and flagella-associated protein 69

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue, rat testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24001-1-AP targets C7orf63 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Ag21205

Product name: Recombinant human C7orf63 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 782-941 aa of BC132771

Sequence: LEAITTASENIGKMVASLQSDIIESQACQDMQNEQKVYAKIQATHKQRELANKSWEDFLARTSNAKTLKKAKRLQEKAIEASRYHKRPQNAIFHQTHIKGLNTMVPSGGVVTVESTPARLVGGPLVDTDIALKKLPIRGGALQRVKAVKIVDAPKKSIPT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 7 open reading frame 63
别名 CFAP69, Cilia- and flagella-associated protein 69
计算分子量 941 aa, 106 kDa
观测分子量110 kDa
GenBank蛋白编号BC132771
基因名称 C7orf63
Gene ID (NCBI) 79846
RRIDAB_3085719
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDA5D8W1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for C7orf63 antibody 24001-1-APDownload protocol
IHC protocol for C7orf63 antibody 24001-1-APDownload protocol
WB protocol for C7orf63 antibody 24001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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