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验证数据展示

  • WB analysis using 31587-1-AP

    WB analysis using 31587-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31587-1-AP (C11orf68 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 31587-1-AP

    WB analysis of A431 using 31587-1-AP

    WB result of C11orf68 antibody (31587-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-C11orf68 transfected A431 cells.

  • IHC staining of human stomach cancer using 31587-1-AP

    IHC staining of human stomach cancer using 31587-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31587-1-AP (C11orf68 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

C11orf68 Polyclonal antibody

C11orf68 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31587-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

BLES03, P5326, Protein p5326, UPF0696 protein C11orf68

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat pancreas tissue, A431 cells, mouse liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31587-1-AP targets C11orf68 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag36023

Product name: Recombinant human C11orf68 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-251 aa of BC010512

Sequence: MEPGEELEEEGSPGGREDGFTAEHLAAEAMAADMDPWLVFDARTTPATELDAWLAKYPPSQVTRYGDPGSPNSEPVGWIAVYGQGYSPNSGDVQGLQAAWEALQTSGRPITPGTLRQLAITHHVLSGKWLMHLAPGFKLDHAWAGIARAVVEGRLQVAKVSPRAKEGGRQVICVYTDDFTDRLGVLEADSAIRAAGIKCLLTYKPDVYTYLGIYRANRWHLCPTLYESRFQLGGSARGSRVLDRANNVELT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 11 open reading frame 68
别名 BLES03, P5326, Protein p5326, UPF0696 protein C11orf68
观测分子量36 kDa
GenBank蛋白编号BC010512
基因名称 C11orf68
Gene ID (NCBI) 83638
RRIDAB_3670046
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9H3H3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BLES03 (basophilic leukemia-expressed protein 03) is a human protein of unknown function. Recent studies have shown that BLES03 is a novel eukaryotic phosphothreonine cleavage enzyme involved in the biosynthesis of dehydrogenated amino acids.The crystal structure of BLES03 has been resolved by single-wavelength anomalous scattering (SAD) with a resolution of 2.5 Å.

实验方案

Product Specific Protocols
IHC protocol for C11orf68 antibody 31587-1-APDownload protocol
WB protocol for C11orf68 antibody 31587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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