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验证数据展示

  • WB analysis using 11859-1-AP (same clone as 11859-1-PBS)

    WB analysis using 11859-1-AP (same clone as 11859-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11859-1-AP (BRD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 11859-1-AP (same clone as 11859-1-PBS)

    IHC staining of human prostate cancer using 11859-1-AP (same clone as 11859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11859-1-AP (BRD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11859-1-AP (same clone as 11859-1-PBS)

    IP experiment of HeLa using 11859-1-AP (same clone as 11859-1-PBS)

    IP result of anti-BRD3 (IP:11859-1-AP, 4ug; Detection:11859-1-AP 1:500) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11859-1-AP (same clone as 11859-1-PBS)

    IF Staining of HeLa using 11859-1-AP (same clone as 11859-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11859-1-AP (BRD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 11859-1-AP (same clone as 11859-1-PBS)

    IF Staining of mouse testis using 11859-1-AP (same clone as 11859-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using BRD3 antibody (11859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11859-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

BRD3 Polyclonal antibody, PBS Only

BRD3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 11859-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

别名

Bromodomain-containing protein 3, KIAA0043, ORFX, RING3 like protein, RING3L

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

11859-1-PBS targets BRD3 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2433

Product name: Recombinant human BRD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-400 aa of BC032124

Sequence: MSTATTVAPAGIPATPGPVNPPPPEVSNPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFYQPVDAIKLNLPDYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTDDIVLMAQALEKIFLQKVAQMPQEEVELLPPAPKGKGRKPAAGAQSAGTQQVAAVSSVSPATPFQSVPPTVSQTPVIAATPVPTITANVTSVPVPPAAAPPPPATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSDPKQAKVVARRESGGRPIKPPKKDLEDGEVPQHAGKKGKLSEHLRYCDSILREMLSKKHAAYAWPFYKPVDAEALELHDYHDIIKHPMDLSTVKRKMDGREYPDAQGFAADVRLMFSNCYKYNPPDHEVVAM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bromodomain containing 3
别名 Bromodomain-containing protein 3, KIAA0043, ORFX, RING3 like protein, RING3L
计算分子量 556 aa, 61 kDa
观测分子量 95-105 kDa
GenBank蛋白编号BC032124
基因名称 BRD3
Gene ID (NCBI) 8019
RRIDAB_2065902
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15059
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BRD3 was belongs to the BET family that a small group of unique, highly conserved bromodomain-containing genes with four members in mouse and human. There are BRD2, BRD3, BRD4 and BRDT. And BET family proteins all localize in the nucleus, and contain two tandems N-terminal BRDs, an extra-terminal (ET) domain and a more divergent C-terminal recruitment domain (PMID:25849938). BRD2, BRD3, and BRD4 play key roles in transcription of genes required for both inflammation and cancer. Northern hybridization revealed that BRD3 is most abundant in testis, ovary, placenta, uterus, and brain(PMID:15261828; PMID:27451123). The antibody could recognize the common about 100 kDa protein in applications as the papers (PMID:29437854; 22595521).

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