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验证数据展示

  • IHC staining of human heart using 19087-1-AP (same clone as 19087-1-PBS)

    IHC staining of human heart using 19087-1-AP (same clone as 19087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19087-1-AP (BMPR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 19087-1-AP (same clone as 19087-1-PBS)

    IHC staining of human heart using 19087-1-AP (same clone as 19087-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 19087-1-AP (BMPR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • FC experiment of PC-3 using 19087-1-AP (same clone as 19087-1-PBS)

    FC experiment of PC-3 using 19087-1-AP (same clone as 19087-1-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.4 ug BMPR2 Polyclonal antibody (19087-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • WB analysis of human heart using 19087-1-AP (same clone as 19087-1-PBS)

    WB analysis of human heart using 19087-1-AP (same clone as 19087-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 19087-1-AP (BMPR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 19087-1-AP (same clone as 19087-1-PBS)

    IP experiment of mouse brain using 19087-1-AP (same clone as 19087-1-PBS)

    IP result of anti-BMPR2 (IP:19087-1-AP, 4ug; Detection:19087-1-AP 1:1500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 19087-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

BMPR2 Polyclonal antibody, PBS Only

BMPR2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 19087-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

BMPR-2, BMPR II, BMPR 2, BMP type-2 receptor, BMP type II receptor

缓冲液配方: 
偶联物:  Unconjugated
规格价格库存

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产品信息

19087-1-PBS targets BMPR2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5868

Product name: Recombinant human BMPR2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 172-504 aa of BC052985

Sequence: YRMLTGDRKQGLHSMNMMEAAASEPSLDLDNLKLLELIGRGRYGAVYKGSLDERPVAVKVFSFANRQNFINEKNIYRVPLMEHDNIARFIVGDERVTADGRMEYLLVMEYYPNGSLCKYLSLHTSDWVSSCRLAHSVTRGLAYLHTELPRGDHYKPAISHRDLNSRNVLVKNDGTCVISDFGLSMRLTGNRLVRPGEEDNAAISEVGTIRYMAPEVLEGAVNLRDCESALKQVDMYALGLIYWEIFMRCTDLFPGESVPEYQMAFQTEVGNHPTFEDMQVLVSREKQRPKFPEAWKENSLAVRSLKETIEDCWDQDAEARLTAQCAEERMAEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bone morphogenetic protein receptor, type II (serine/threonine kinase)
别名 BMPR-2, BMPR II, BMPR 2, BMP type-2 receptor, BMP type II receptor
计算分子量 115 kDa
观测分子量 120 kDa
GenBank蛋白编号BC052985
基因名称 BMPR2
Gene ID (NCBI) 659
RRIDAB_10603355
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13873
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BMPs (bone morphogenetic protein) are involved in endochondral bone formation and embryogenesis. BMPR2 encodes a member of the (BMP) receptor family of transmembrane serine/threonine kinases. It is a widely expressed receptor, with high mRNA expression during development in many tissues. The dimer of BMPR2 receptors (70-80 kD) forms a complex with a dimer of type I BMPR1(50-55 kD) in signal transduction. This antibody recognizes endogenous levels of total BMPR2 protein, including 115 kD precursor, 75 kD mature form, and 150 kD dimer.

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