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验证数据展示

  • WB analysis using 31090-1-AP

    WB analysis using 31090-1-AP

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 31090-1-AP (Atox1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 31090-1-AP

    IHC staining of mouse heart using 31090-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31090-1-AP (Atox1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Atox1 Polyclonal antibody

Atox1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31090-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

HAH1, Atx1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNCI-H1299 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31090-1-AP targets Atox1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35290

Product name: Recombinant mouse Atox1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-68 aa of NM_009720

Sequence: MPKHEFSVDMTCEGCAEAVSRVLNKLGGVEFNIDLPNKKVCIDSEHSSDTLLATLNKTGKAVSYLGPK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATX1 (antioxidant protein 1) homolog 1 (yeast)
别名 HAH1, Atx1
计算分子量7 kDa
观测分子量7-10 kDa
GenBank蛋白编号NM_009720
基因名称 Atox1
Gene ID (NCBI) 11927
RRIDAB_3669848
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO08997
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Antioxidant 1 copper chaperone (Atox1) is a small cytosolic protein containing an MBS (metal binding site) and a potential NLS (nuclear localisation signal). It plays an essential role in copper homeostasis by facilitating the transfer of copper to the trans-Golgi. Atox1 has been implicated in a wide range of diseases, including many neurodegenerative, cancer and metabolic disorders.

实验方案

Product Specific Protocols
WB protocol for Atox1 antibody 31090-1-APDownload protocol
IHC protocol for Atox1 antibody 31090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols