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验证数据展示

  • WB analysis using 31090-1-AP

    WB analysis using 31090-1-AP

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 31090-1-AP (Atox1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 31090-1-AP

    IHC staining of mouse heart using 31090-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31090-1-AP (Atox1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Atox1 Polyclonal antibody

Atox1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31090-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

HAH1, Atx1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNCI-H1299 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31090-1-AP targets Atox1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Atox1 fusion protein Ag35290 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATX1 (antioxidant protein 1) homolog 1 (yeast)
别名 HAH1, Atx1
计算分子量7 kDa
观测分子量7-10 kDa
GenBank蛋白编号NM_009720
基因名称 Atox1
Gene ID (NCBI) 11927
RRIDAB_3669848
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO08997
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Antioxidant 1 copper chaperone (Atox1) is a small cytosolic protein containing an MBS (metal binding site) and a potential NLS (nuclear localisation signal). It plays an essential role in copper homeostasis by facilitating the transfer of copper to the trans-Golgi. Atox1 has been implicated in a wide range of diseases, including many neurodegenerative, cancer and metabolic disorders.

实验方案

Product Specific Protocols
WB protocol for Atox1 antibody 31090-1-APDownload protocol
IHC protocol for Atox1 antibody 31090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols