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验证数据展示

  • WB analysis of HEK-293 using 11401-1-AP (same clone as 11401-1-PBS)

    WB analysis of HEK-293 using 11401-1-AP (same clone as 11401-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11401-1-AP (alpha Adaptin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11401-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 11401-1-AP (same clone as 11401-1-PBS)

    IHC staining of human ovary tumor using 11401-1-AP (same clone as 11401-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11401-1-AP (alpha Adaptin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11401-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AP2A1 antibody included
  • IF Staining of HeLa using 11401-1-AP (same clone as 11401-1-PBS)

    IF Staining of HeLa using 11401-1-AP (same clone as 11401-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alpha Adaptin antibody (11401-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11401-1-PBS in a different storage buffer formulation.

Alpha Adaptin Polyclonal antibody, PBS Only

Alpha Adaptin Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11401-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AP2A1, Alpha1 adaptin, Alpha adaptin A, ADTAA, Adaptor-related protein complex 2 subunit alpha-1

缓冲液配方: 
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy AP2A1 Ready-To-Use IHC Kit. AP2A1 primary antibody included.

产品信息

11401-1-PBS targets Alpha Adaptin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1927

Product name: Recombinant human AP2A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 677-982 aa of BC014214

Sequence: GAGNLLVDVFDGPAAQPSLGPTPEEAFLSPGPEDIGPPIPEADELLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPGDREDTRVWGMPGTFLRPFVFLFLFICCCLHSGGLGGVPLPPFPPQAQRGEGPGKWMSPPLPPHPVVAPPTPSPSRGCVLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 2, alpha 1 subunit
别名 AP2A1, Alpha1 adaptin, Alpha adaptin A, ADTAA, Adaptor-related protein complex 2 subunit alpha-1
计算分子量 977 aa, 108 kDa
观测分子量 108 kDa
GenBank蛋白编号BC014214
基因名称 AP2A1
Gene ID (NCBI) 160
RRIDAB_2056316
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95782
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AP2A1 encodes adaptor protein complex 2 (AP-2) subunit alpha-1, which is a 100 kDa coated vesicle protein. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 is involved in clathrin-dependent endocytosis.

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