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验证数据展示

  • WB analysis of HeLa using 11313-2-AP (same clone as 11313-2-PBS)

    WB analysis of HeLa using 11313-2-AP (same clone as 11313-2-PBS)

    WB result of Alpha Actinin antibody (11313-2-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Actinin transfected HeLa cells. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • WB analysis using 11313-2-AP (same clone as 11313-2-PBS)

    WB analysis using 11313-2-AP (same clone as 11313-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11313-2-AP (Alpha Actinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 11313-2-AP (same clone as 11313-2-PBS)

    IHC staining of human heart using 11313-2-AP (same clone as 11313-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Alpha Actinin antibody included
  • IHC staining of human heart using 11313-2-AP (same clone as 11313-2-PBS)

    IHC staining of human heart using 11313-2-AP (same clone as 11313-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11313-2-AP (alpha Actinin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Alpha Actinin antibody included
  • IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

    IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 11313-2-AP (alpha Actinin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 66219-1-Ig (N-cadherin) in red. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 11313-2-AP (same clone as 11313-2-PBS)

    IHC staining of human colon using 11313-2-AP (same clone as 11313-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11313-2-AP (Alpha Actinin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Alpha Actinin antibody included
  • IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

    IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed mouse heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 11313-2-AP (same clone as 11313-2-PBS)

    IF Staining of mouse skeletal muscle using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse skeletal muscle using 11313-2-AP (same clone as 11313-2-PBS)

    IF Staining of mouse skeletal muscle using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

    IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

    IF Staining of mouse heart using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • IF Staining of rat heart using 11313-2-AP (same clone as 11313-2-PBS)

    IF Staining of rat heart using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • IF Staining of rat heart using 11313-2-AP (same clone as 11313-2-PBS)

    IF Staining of rat heart using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using Alpha Actinin antibody (11313-2-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • IF Staining of HSkMC using 11313-2-AP (same clone as 11313-2-PBS)

    IF Staining of HSkMC using 11313-2-AP (same clone as 11313-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HSkMC cells using Alpha Actinin antibody (11313-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 11313-2-AP (same clone as 11313-2-PBS)

    FC experiment of A431 using 11313-2-AP (same clone as 11313-2-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actinin (11313-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 11313-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Alpha Actinin Polyclonal antibody, PBS Only

Alpha Actinin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 11313-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

ACTN1, Alpha actinin 1, actin, actinin, alpha 1, ACTN 1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Alpha Actinin Ready-To-Use IHC Kit. Alpha Actinin primary antibody included.

产品信息

11313-2-PBS targets Alpha Actinin in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1859

Product name: Recombinant human a-Actinin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 543-892 aa of BC015766

Sequence: EIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 actinin, alpha 1
别名 ACTN1, Alpha actinin 1, actin, actinin, alpha 1, ACTN 1
计算分子量 103 kDa
观测分子量 100-105 kDa
GenBank蛋白编号BC015766
基因名称 Alpha Actinin
Gene ID (NCBI) 87
RRIDAB_2223815
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP12814
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

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