Dot blot analysis was used to confirm the specificity of 83041-6-RR Acetyl-Histone H2A (Lys15) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 83041-6-PBS in a different storage buffer formulation.

Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83041-6-RR (Acetyl-Histone H2A (Lys15) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83041-6-PBS in a different storage buffer formulation.