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AbFlex Histone H3K4me2 antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Wide Range Predicted

Applications

ChIP, ChIP-Seq, WB

Cat No : 91321,91322 91321

Synonyms

abflex, histone H3, histone, H3, histone-H3, histoneH3, pAb, monoclonal, H3K4me2, Histone H3 dimethyl Lys4, Histone H3 dimethyl Lysine 4, Histone H3 dimethyl Lys 4, Histone H3 dimethyl K4, Histone H3 dimethyl K 4, Histone H3 di-methyl Lys4, Histone H3 di-methyl Lysine 4, Histone H3 di-methyl Lys 4, Histone H3 di-methyl K4, Histone H3 di-methyl K 4, Histone H3 2Me Lys4, Histone H3 2Me Lysine 4, Histone H3 2Me Lys 4, Histone H3 2Me K4, Histone H3 2Me K 4, Histone H3 Me2 Lys4, Histone H3 Me2 Lysine 4, Histone H3 Me2 Lys 4, Histone H3 Me2 K4, Histone H3 Me2 K 4, antibody, antibodies, MABI, Clone MABI 0303, chip, chromatin immunoprecipitation, H3K4Me2, peptest, 61315

验证数据展示

  • AbFlex Histone H3K4me2 recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of HeLa cell chromatin and 5 ug of AbFlex Histone H3K4me2 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 14.2 million sequence tags were mapped to identify Histone H3K4me2 binding sites on chromosome 7.

    AbFlex Histone H3K4me2 recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of HeLa cell chromatin and 5 ug of AbFlex Histone H3K4me2 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 14.2 million sequence tags were mapped to identify Histone H3K4me2 binding sites on chromosome 7.

  • AbFlex Histone H3K4me2 specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3K4me2 antibody was applied to Active Motif's MODified Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.

    AbFlex Histone H3K4me2 specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3K4me2 antibody was applied to Active Motif's MODified Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.

  • AbFlexHistone H3K4me2 specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3K4me2 antibody was applied to Active Motif's MODified Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.

    AbFlexHistone H3K4me2 specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3K4me2 antibody was applied to Active Motif's MODified Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen.

产品信息

Tested Applications ChIP, ChIP-Seq, WB

Applications Validated by Active Motif: ChIP-Seq: 5 ug/ml WB*: 0.5 - 2 ug/ml DB: 1 ug/ml *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
Tested Reactivity Human, Wide Range Predicted
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
ModificationMethylated
Immunogen This antibody was raised against a synthetic peptide containing dimethyl Lys4 of human histone H3.
Full Name AbFlex Histone H3K4me2 antibody (rAb)
Synonyms abflex, histone H3, histone, H3, histone-H3, histoneH3, pAb, monoclonal, H3K4me2, Histone H3 dimethyl Lys4, Histone H3 dimethyl Lysine 4, Histone H3 dimethyl Lys 4, Histone H3 dimethyl K4, Histone H3 dimethyl K 4, Histone H3 di-methyl Lys4, Histone H3 di-methyl Lysine 4, Histone H3 di-methyl Lys 4, Histone H3 di-methyl K4, Histone H3 di-methyl K 4, Histone H3 2Me Lys4, Histone H3 2Me Lysine 4, Histone H3 2Me Lys 4, Histone H3 2Me K4, Histone H3 2Me K 4, Histone H3 Me2 Lys4, Histone H3 Me2 Lysine 4, Histone H3 Me2 Lys 4, Histone H3 Me2 K4, Histone H3 Me2 K 4, antibody, antibodies, MABI, Clone MABI 0303, chip, chromatin immunoprecipitation, H3K4Me2, peptest, 61315
Molecular weight 17 kDa
GenBank accession numberNP_003522
Purification Method Protein A Chromatography
Buffer Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex H3K4me2 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.