AbFlex Histone H3.3 antibody (rAb)
Host / Isotype
Mouse / IgG2a
Cat No : 91191,91192 91191
Applications Validated by Active Motif: ChIP-Seq: 4 ug per ChIP WB*: 2 ug/ml dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
|Host / Isotype||Mouse / IgG2a|
|Immunogen||This antibody was raised against a peptide corresponding to amino acids 21-39 of human Histone H3.3.|
|Full Name||AbFlex Histone H3.3 antibody (rAb)|
|Synonyms||Histone H3, globular domain, nucleosome, chromatin, antibody, antibodies, polyclonal, histone H3, histone, H3, histone-H3, histoneH3, c-terminal, antibody, antibodies, chip, chromatin immunoprecipitation, chromatin immunoprecipitation, chip, AbFlex, recombinant antibody|
|Molecular weight||17 kDa|
|GenBank accession number||NP_002908||RRID||AB_2793796||Purification Method||Protein A Chromatography|
|Buffer||Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.|
|Storage||Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.|
AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3.3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription. Aberrant localization of these variants is also known to correlate with certain cancers.