AbFlex EED antibody (rAb)
Host / Isotype
Mouse / IgG2a
ChIP, ChIP-Seq, ELISA
Cat No : 91135,91136 91135
ChIP, ChIP-Seq, ELISA
Applications Validated by Active Motif: ChIP-Seq: 4 ug per ChIP Bead-based ELISA: 4 - 26 ng/ml AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61203.
|Host / Isotype||Mouse / IgG2a|
|Immunogen||This EED antibody was raised against full-length recombinant human EED protein.|
|Full Name||AbFlex EED antibody (rAb)|
|Synonyms||EED, extraembryonic ectoderm, Polycomb, PRC2, SUZ12, WD-40, silencing, embryonic stem cells, escs, monoclonal, clone 41d, stem cell, stem cells, recombinant, rAb, rec Ab, AbFlex|
|Molecular weight||57 kDa|
|GenBank accession number||NP_003788||RRID||AB_2793786||Purification Method||Ni-NTA|
|Buffer||Purified IgG in 200 mM Hepes pH 7.5, 100 mm NaCl, 50 mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.|
|Storage||Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.|
AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex EED antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. EED (extraembryonic ectoderm) is a component of the Polycomb group (PcG) multiprotein complex PRC2. The PRC2 complex also contains EZH2 and SUZ12 and is recruited to Polycomb response elements and results in the methylation of histone H3 and lysine 27 and the silencing of gene expression. PRC2 initiates repression of the Hox genes during development and is involved in the maintenance of stem cell pluripotency. EED is a member of the superfamily of WD-40 repeat protein family and is required for PRC2-mediated H3K27 methylation. The PRC2 complex may also recruit platform DNMTs, thereby linking two systems of epigenetic repression.