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AbFlex EED antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

ChIP, ChIP-Seq, ELISA

Cat No : 91135,91136 91135

Synonyms

EED, extraembryonic ectoderm, Polycomb, PRC2, SUZ12, WD-40, silencing, embryonic stem cells, escs, monoclonal, clone 41d, stem cell, stem cells, recombinant, rAb, rec Ab, AbFlex

验证数据展示

  • AbFlex EED Antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug chromatin from an acute large B-cell lymphoma cell line and 4 ug of EED antibody. ChIP DNA was sequenced on the Illumina HiSeq and 25 million sequence tags were mapped to identify EED binding sites. The image shows binding across a region of chromosome 6.

    AbFlex EED Antibody (rAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug chromatin from an acute large B-cell lymphoma cell line and 4 ug of EED antibody. ChIP DNA was sequenced on the Illumina HiSeq and 25 million sequence tags were mapped to identify EED binding sites. The image shows binding across a region of chromosome 6.

  • AbFlex EED antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlexTM EED antibody (rAb) for EED. Various proteins were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlexTM EED antibody (rAb). Protein-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.

    AbFlex EED antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlexTM EED antibody (rAb) for EED. Various proteins were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlexTM EED antibody (rAb). Protein-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.

产品信息

Tested Applications ChIP, ChIP-Seq, ELISA

Applications Validated by Active Motif: ChIP-Seq: 4 ug per ChIP Bead-based ELISA: 4 - 26 ng/ml AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61203.
Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen This EED antibody was raised against full-length recombinant human EED protein.
Full Name AbFlex EED antibody (rAb)
Synonyms EED, extraembryonic ectoderm, Polycomb, PRC2, SUZ12, WD-40, silencing, embryonic stem cells, escs, monoclonal, clone 41d, stem cell, stem cells, recombinant, rAb, rec Ab, AbFlex
Molecular weight 57 kDa
GenBank accession numberNP_003788
RRIDAB_2793786
Purification Method Ni-NTA
Buffer Purified IgG in 200 mM Hepes pH 7.5, 100 mm NaCl, 50 mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex EED antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. EED (extraembryonic ectoderm) is a component of the Polycomb group (PcG) multiprotein complex PRC2. The PRC2 complex also contains EZH2 and SUZ12 and is recruited to Polycomb response elements and results in the methylation of histone H3 and lysine 27 and the silencing of gene expression. PRC2 initiates repression of the Hox genes during development and is involved in the maintenance of stem cell pluripotency. EED is a member of the superfamily of WD-40 repeat protein family and is required for PRC2-mediated H3K27 methylation. The PRC2 complex may also recruit platform DNMTs, thereby linking two systems of epigenetic repression.