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AbFlex CHD3 antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Drosophila

Applications

IP, WB

Cat No : 91273,91274 91273

Synonyms

CHD3, Chromodomain Helicase DNA Binding Protein 3, ATP-Dependent Helicase CHD3, Mi-2a, HZFH, Mi2 ALPHA, Chromodomain-Helicase-DNA-Binding Protein 3, ZFH, Zinc-Finger Helicase (Snf2-Like), Mi-2 Autoantigen 240 KDa Protein, Zinc Finger Helicase, Mi2-Alpha, CHD-3, ATP-dependent chromatin remodeler, nucleosome remodeling and deacetylation, NuRD, CHD,

验证数据展示

  • AbFlex CHD3 antibody (rAb) tested by Western blot. 15 ug of Drosophila Schneider Cell nuclear extract was run on SDS-PAGE and probed with AbFlex CHD3 antibody at 0.5 ug/ml.

    AbFlex CHD3 antibody (rAb) tested by Western blot. 15 ug of Drosophila Schneider Cell nuclear extract was run on SDS-PAGE and probed with AbFlex CHD3 antibody at 0.5 ug/ml.

  • AbFlex CHD3 antibody (rAb) tested antibody tested by immunoprecipitation. Drosophila Schneider cell nuclear extract was immunoprecipitated using 4 ug of AbFlex CHD3 antibody (Lane 1) or an unrelated AbFlex antibody (Lane 2). Protein from IP was probed by Western blot using AbFlex CHD3 antibody (2 ug/ml).

    AbFlex CHD3 antibody (rAb) tested antibody tested by immunoprecipitation. Drosophila Schneider cell nuclear extract was immunoprecipitated using 4 ug of AbFlex CHD3 antibody (Lane 1) or an unrelated AbFlex antibody (Lane 2). Protein from IP was probed by Western blot using AbFlex CHD3 antibody (2 ug/ml).

产品信息

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 4 ug per IP WB: 0.5 - 2 ug/ml For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background. Individual optimization may be required. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61469.
Tested Reactivity Drosophila
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen This antibody was raised against a synthetic peptide corresponding to amino acid residues 865-885 of Drosophila.
Full Name AbFlex CHD3 antibody (rAb)
Synonyms CHD3, Chromodomain Helicase DNA Binding Protein 3, ATP-Dependent Helicase CHD3, Mi-2a, HZFH, Mi2 ALPHA, Chromodomain-Helicase-DNA-Binding Protein 3, ZFH, Zinc-Finger Helicase (Snf2-Like), Mi-2 Autoantigen 240 KDa Protein, Zinc Finger Helicase, Mi2-Alpha, CHD-3, ATP-dependent chromatin remodeler, nucleosome remodeling and deacetylation, NuRD, CHD,
Molecular weight 103 kDa
GenBank accession numberNP_649111
RRIDAB_2793829
Purification Method Protein A Chromatography
Buffer Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex CHD3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. CHD3 (chromodomain helicase DNA binding protein 3) is a component of the CHD family of ATP-dependent chromatin remodelers and forms histone deacetylase NuRD complexes which participate in the remodeling of chromatin by deacetylating histones.