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验证数据展示

  • WB analysis using 68397-1-Ig (same clone as 68397-1-PBS)

    WB analysis using 68397-1-Ig (same clone as 68397-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68397-1-Ig (ATPBD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68397-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 68397-1-Ig (same clone as 68397-1-PBS)

    WB analysis of K-562 using 68397-1-Ig (same clone as 68397-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 68397-1-Ig (ATPBD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68397-1-PBS in a different storage buffer formulation.

ATPBD4 Monoclonal antibody, PBS Only

ATPBD4 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68397-1-PBS

产品说明书

CloneNo. 2B12E1

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, Indirect ELISA

别名

ATP binding domain 4, ATPBD4, DPH6

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68397-1-PBS targets ATPBD4 in WB, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag33218

Product name: Recombinant human ATPBD4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-259 aa of BC008485

Sequence: GGKDSCYNMMQCIAAGHQIVALANLRPAENQVGSDELDSYMYQTVGHHAIDLYAEAMALPLYRRTIRGRSLDTRQVYTKCEGDEVEDLYELLKLVKEKEEVEGISVGAILSDYQRIRVENVCKRLNLQPLAYLWQRNQEDLLREMISSNIQAMIIKVAALGLDPDKHLGKTLDQMEPYLIELSKKYGVHVCGEGGEYETFTLDCPLFKKKIIVDSSEVVIHSADAFAPVAYLRFLELHLEDKVSSVPDNYRTSNYIYNF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP binding domain 4
别名 ATP binding domain 4, ATPBD4, DPH6
计算分子量 267 aa, 30 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC008485
基因名称 ATPBD4
Gene ID (NCBI) 89978
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ7L8W6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Diphthamide is a highly modified histidine in the transcription factor EEF2 that is conserved from yeast to human. ATPBD4, also known as DPH6, belongs to the Diphthine--ammonia ligase family. DPH6 amidase may catalyze the last step of diphthamide biosynthesis using ammonium and ATP to play a role in catalyzing the final step of diphthamide biosynthesis, amidation of diphthine to diphthamide (PubMed:23169644).

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