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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98589-1-RR

    FC experiment of human peripheral blood leukocytes using 98589-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human ATP1B3 Rabbit RecAb (98589-1-RR, Clone:251377D4) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98589-1-RR

    FC experiment of human peripheral blood leukocytes using 98589-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human ATP1B3 Rabbit RecAb (98589-1-RR, Clone:251377D4)(red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)(blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity of 98589-1-RR

    Affinity of 98589-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98589-1-RR against Human ATP1B3 were performed. The affinity constant is below 1 pM.

Anti-Human ATP1B3 Rabbit Recombinant Antibody

ATP1B3 Uni-rAb® Recombinant Antibody for FC
Cat No. 98589-1-RR

产品说明书

CloneNo. 251377D4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

ATPB 3, ATPB-3, CD298, Sodium/potassium-dependent ATPase subunit beta-3, Sodium/potassium-transporting ATPase subunit beta-3

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inFC receptor blocked human peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy ATP1B3 Ready-To-Use IHC Kit. ATP1B3 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98589-1-RR targets ATP1B3 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

CatNo: Eg4947

Product name: Recombinant Human ATP1B3 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 57-279 aa of BC011835

Sequence: TMWVMLQTLNDEVPKYRDQIPSPGLMVFPKPVTALEYTFSRSDPTSYAGYIEDLKKFLKPYTLEEQKNLTVCPDGALFEQKGPVYVACQFPISLLQACSGMNDPDFGYSQGNPCILVKMNRIIGLKPEGVPRIDCVSKNEDIPNVAVYPHNGMIDLKYFPYYGKKLHVGYLQPLVAVQVSFAPNNTGKEVTVECKIDGSANLKSQDDRDKFLGRVMFKITARA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATPase, Na+/K+ transporting, beta 3 polypeptide
别名 ATPB 3, ATPB-3, CD298, Sodium/potassium-dependent ATPase subunit beta-3, Sodium/potassium-transporting ATPase subunit beta-3
计算分子量 32 kDa
GenBank蛋白编号BC011835
基因名称 ATP1B3
Gene ID (NCBI) 483
RRIDAB_3746200
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP54709
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).

实验方案

Product Specific Protocols
FC protocol for ATP1B3 antibody 98589-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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