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验证数据展示

  • IF Staining of mouse liver using CL488-84047

    IF Staining of mouse liver using CL488-84047

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CoraLite® Plus 488 APOB antibody (CL488-84047, Clone: 240884H4 ) at dilution of 1:100. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using CL488-84047

    IF Staining of mouse liver using CL488-84047

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CoraLite® Plus 488 APOB antibody (CL488-84047, Clone: 240884H4 ) at dilution of 1:100. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated APOB Recombinant antibody

APOB Recombinant Antibody for IF-P
Cat No. CL488-84047

产品说明书

CloneNo. 240884H4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse liver tissue
For other applications, we recommend the unconjugated version of this antibody, 84047-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84047 targets APOB in IF-P applications and shows reactivity with human, mouse samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 apolipoprotein B (including Ag(x) antigen)
别名
计算分子量 516 kDa
观测分子量150-250 kDa, 400-520 kDa
GenBank蛋白编号NM_000384
基因名称 APOB
Gene ID (NCBI) 338
RRIDAB_3673333
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04114
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 APOB antibody CL488-84047Download protocol
Standard Protocols
Click here to view our Standard Protocols