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验证数据展示

  • WB analysis using 68388-1-Ig (same clone as 68388-1-PBS)

    WB analysis using 68388-1-Ig (same clone as 68388-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68388-1-Ig (ANKRD54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68388-1-PBS in a different storage buffer formulation.

  • WB analysis of U2OS using 68388-1-Ig (same clone as 68388-1-PBS)

    WB analysis of U2OS using 68388-1-Ig (same clone as 68388-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 68388-1-Ig (ANKRD54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68388-1-PBS in a different storage buffer formulation.

ANKRD54 Monoclonal antibody, PBS Only

ANKRD54 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68388-1-PBS

产品说明书

CloneNo. 1E9A9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, Indirect ELISA

别名

ANKRD54, ankyrin repeat domain 54

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® ANKRD54 Ready-To-Use IHC Kit. ANKRD54 primary antibody included.

产品信息

68388-1-PBS targets ANKRD54 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag22772

Product name: Recombinant human ANKRD54 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 201-300 aa of BC066909

Sequence: RVDALDRAGRTPLHLAKSKLNILQEGHAQCLEAVRLEVKQIIHMLREYLERLGQHEQRERLDDLCTRLQMTSTKEQVDEVTDLLASFTSLSLQMQSMEKR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ankyrin repeat domain 54
别名 ANKRD54, ankyrin repeat domain 54
计算分子量 300 aa, 33 kDa
观测分子量 33-35 kDa
GenBank蛋白编号BC066909
基因名称 ANKRD54
Gene ID (NCBI) 129138
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ6NXT1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ankyrin repeat domain 54 (ANKRD54)/Liar is a nuclear-cytoplasmic shuttling adaptor that interacts with Lyn, Btk, Txk, and HCLS1. Activation of PKC kinases promoted nuclear export and phosphorylation of Ankrd54, and increased interaction and cytoplasmic co-localization with Lyn.

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