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ANKRD54 Monoclonal antibody

ANKRD54 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E9A9

Cat No : 68388-1-Ig

Print datasheet

Synonyms

ANKRD54, ankyrin repeat domain 54

验证数据展示

  • WB analysis using 68388-1-Ig

    WB analysis using 68388-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68388-1-Ig (ANKRD54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of U2OS using 68388-1-Ig

    WB analysis of U2OS using 68388-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 68388-1-Ig (ANKRD54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, U2OS cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68388-1-Ig targets ANKRD54 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen ANKRD54 fusion protein Ag22772 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ankyrin repeat domain 54
Synonyms ANKRD54, ankyrin repeat domain 54
Calculated Molecular Weight 300 aa, 33 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberBC066909
Gene Symbol ANKRD54
Gene ID (NCBI) 129138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6NXT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ankyrin repeat domain 54 (ANKRD54)/Liar is a nuclear-cytoplasmic shuttling adaptor that interacts with Lyn, Btk, Txk, and HCLS1. Activation of PKC kinases promoted nuclear export and phosphorylation of Ankrd54, and increased interaction and cytoplasmic co-localization with Lyn.

实验方案

Product Specific Protocols
WB protocol for ANKRD54 antibody 68388-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols