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验证数据展示

  • WB analysis using 68388-1-Ig

    WB analysis using 68388-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68388-1-Ig (ANKRD54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis of U2OS using 68388-1-Ig

    WB analysis of U2OS using 68388-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 68388-1-Ig (ANKRD54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

ANKRD54 Monoclonal antibody

ANKRD54 Monoclonal Antibody for WB, ELISA
Cat No. 68388-1-Ig

产品说明书

CloneNo. 1E9A9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

WB, ELISA

别名

ANKRD54, ankyrin repeat domain 54

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, U2OS cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68388-1-Ig targets ANKRD54 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 ANKRD54 fusion protein Ag22772 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ankyrin repeat domain 54
别名 ANKRD54, ankyrin repeat domain 54
计算分子量 300 aa, 33 kDa
观测分子量 33-35 kDa
GenBank蛋白编号BC066909
基因名称 ANKRD54
Gene ID (NCBI) 129138
RRIDAB_3085106
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ6NXT1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ankyrin repeat domain 54 (ANKRD54)/Liar is a nuclear-cytoplasmic shuttling adaptor that interacts with Lyn, Btk, Txk, and HCLS1. Activation of PKC kinases promoted nuclear export and phosphorylation of Ankrd54, and increased interaction and cytoplasmic co-localization with Lyn.

实验方案

Product Specific Protocols
WB protocol for ANKRD54 antibody 68388-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols