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ANKIB1 Polyclonal antibody

ANKIB1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 30467-1-AP

Print datasheet

Synonyms

ANKIB1, DKFZp434A0225, FLJ33123, KIAA1386

验证数据展示

  • WB analysis using 30467-1-AP

    WB analysis using 30467-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30467-1-AP (ANKIB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 30467-1-AP

    IHC staining of mouse kidney using 30467-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 30467-1-AP

    IHC staining of mouse kidney using 30467-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse placenta using 30467-1-AP

    IHC staining of mouse placenta using 30467-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse placenta using 30467-1-AP

    IHC staining of mouse placenta using 30467-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30467-1-AP (ANKIB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 30467-1-AP

    IP experiment of HeLa using 30467-1-AP

    IP result of anti-ANKIB1 (IP:30467-1-AP, 4ug; Detection:30467-1-AP 1:4000) with HeLa cells lysate 1560 ug.

  • IF Staining of U2OS using 30467-1-AP

    IF Staining of U2OS using 30467-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ANKIB1 antibody (30467-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A431 using 30467-1-AP

    IF Staining of A431 using 30467-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ANKIB1 antibody (30467-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Jurkat cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse kidney tissue, mouse placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, A431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30467-1-AP targets ANKIB1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity Human, mouse
Immunogen ANKIB1 fusion protein Ag33126 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ankyrin repeat and IBR domain containing 1
Synonyms ANKIB1, DKFZp434A0225, FLJ33123, KIAA1386
Calculated Molecular Weight122 kDa
Observed Molecular Weight 122 kDa
GenBank Accession NumberNM_019004.1
Gene Symbol ANKIB1
Gene ID (NCBI) 54467
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2G1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ankyrin repeat and IBR domain-containing protein 1 (ANKIB1) is also named as KIAA1386, and belongs to the RBR family. ANKIB1 might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates. The common biomarkers found within the H and EC region of brain are ZNF621, SLC25A46, RAE1, and ANKIB1. Among these biomarkers, RAE1, ANKIB1, and SLC25A46 have been reported to be prominently involved in several neurodegenerative disorders (PMID:33878365). ANKIB1 is also found to be associated with Cerebral cavernous malformations (PMID:20798775).

实验方案

Product Specific Protocols
WB protocol for ANKIB1 antibody 30467-1-APDownload protocol
IHC protocol for ANKIB1 antibody 30467-1-APDownload protocol
IF protocol for ANKIB1 antibody 30467-1-APDownload protocol
IP protocol for ANKIB1 antibody 30467-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols