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验证数据展示

  • WB analysis using 23938-1-AP

    WB analysis using 23938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23938-1-AP (ANK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 23938-1-AP

    IHC staining of mouse cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of mouse cerebellum using 23938-1-AP

    IHC staining of mouse cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of rat cerebellum using 23938-1-AP

    IHC staining of rat cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of rat cerebellum using 23938-1-AP

    IHC staining of rat cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of human lymphoma using 23938-1-AP

    IHC staining of human lymphoma using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of human stomach cancer using 23938-1-AP

    IHC staining of human stomach cancer using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included

ANK1 Polyclonal antibody

ANK1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23938-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Ankyrin 1, Ankyrin-1, Ankyrin R, ANK-1, ANK 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, mouse liver tissue
Positive IHC detected inhuman stomach cancer tissue, human lymphoma tissue, mouse cerebellum tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ANK1 Ready-To-Use IHC Kit. ANK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23938-1-AP targets ANK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21041

Product name: Recombinant human ANK1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-155 aa of BC030957

Sequence: MWTFVTQLLVTLVLLSFFLVSCQNVMHIVRGSLCFVLKHIHQELDKELGESEDLSDDEETISTRVVRRRVFLKGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQIDLSSADAAQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin 1, erythrocytic
别名 Ankyrin 1, Ankyrin-1, Ankyrin R, ANK-1, ANK 1
计算分子量 155 aa, 18 kDa
观测分子量 190-250 kDa
GenBank蛋白编号BC030957
基因名称 ANK1
Gene ID (NCBI) 286
RRIDAB_3085717
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDP16157
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ankyrin-1 (ANK1) is also named as Ankyrin-R and Erythrocyte ankyrin. The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex (PMID:18440852). ANK1 can attache integral membrane proteins to cytoskeletal elements. And it binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin.(PMID:12456646).

实验方案

Product Specific Protocols
IHC protocol for ANK1 antibody 23938-1-APDownload protocol
WB protocol for ANK1 antibody 23938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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