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验证数据展示

  • WB analysis using 23938-1-AP

    WB analysis using 23938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23938-1-AP (ANK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 23938-1-AP

    IHC staining of mouse cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of mouse cerebellum using 23938-1-AP

    IHC staining of mouse cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of rat cerebellum using 23938-1-AP

    IHC staining of rat cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of rat cerebellum using 23938-1-AP

    IHC staining of rat cerebellum using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of human lymphoma using 23938-1-AP

    IHC staining of human lymphoma using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included
  • IHC staining of human stomach cancer using 23938-1-AP

    IHC staining of human stomach cancer using 23938-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23938-1-AP (ANK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ANK1 antibody included

ANK1 Polyclonal antibody

ANK1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23938-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Ankyrin 1, ANK, ANK 1, ANK-1, Ankyrin R

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, mouse liver tissue
Positive IHC detected inhuman stomach cancer tissue, human lymphoma tissue, mouse cerebellum tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ANK1 Ready-To-Use IHC Kit. ANK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23938-1-AP targets ANK1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21041

Product name: Recombinant human ANK1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-155 aa of BC030957

Sequence: MWTFVTQLLVTLVLLSFFLVSCQNVMHIVRGSLCFVLKHIHQELDKELGESEDLSDDEETISTRVVRRRVFLKGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQIDLSSADAAQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin 1, erythrocytic
别名 Ankyrin 1, ANK, ANK 1, ANK-1, Ankyrin R
计算分子量 155 aa, 18 kDa
观测分子量 190-250 kDa
GenBank蛋白编号BC030957
基因名称 ANK1
Gene ID (NCBI) 286
RRIDAB_3085717
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDP16157-17
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ankyrin-1 (ANK1) is also named as Ankyrin-R and Erythrocyte ankyrin. The stability and shape of the erythrocyte membrane is provided by the ankyrin-1 complex (PMID:18440852). ANK1 can attache integral membrane proteins to cytoskeletal elements. And it binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin.(PMID:12456646). This target recognizes the C-terminal of Ankyrin-1 and also recognizes ankyrin-1 isoform 5 (206 kDa). It can identify isoforms such as Er5, Er6, Er11, Er12, Er13, Er14, Er16, Mu17, Mu18, Mu19, Mu20, 22, and 23.

实验方案

Product Specific Protocols
IHC protocol for ANK1 antibody 23938-1-APDownload protocol
WB protocol for ANK1 antibody 23938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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